prokaryotic transcription Flashcards
feature that is similar in both transcription and replication
RNA synthesis require DNA topoisomerase action
synthesis of mRNA on DNA template is
unidirection
mechanism that checks to make sure that the right monomer is incorporated into the polymer
RNA synthesis
enzyme that does not require a primer
DNA dependent RNA polymerase
E.coli polymerase have
holoenzyme include sigma factor, require Mg2+ and Zn2+
DNA binding protein that initiates the transcription of bacterial genes is called
sigma factor
sigma subunit of RNA polymerase
specifies the site for transcription
binding of sigma factor depends on the
recognition of the length and spacing of promotor consensus sequence
role of the promotor region of a gene
site where RNA polymerase binds to DNA
pribnow box is centered at
-10 position
mode of action of rifampicin in E.coli is through
initiation of transcription and binding of RNA polymerase to DNA template
TATA box plays a key role in ascending active transcription
binding with TATA box binding protein
transcription factors bind to TATA box
TFIID