prokaryotic transcription Flashcards

1
Q

feature that is similar in both transcription and replication

A

RNA synthesis require DNA topoisomerase action

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2
Q

synthesis of mRNA on DNA template is

A

unidirection

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3
Q

mechanism that checks to make sure that the right monomer is incorporated into the polymer

A

RNA synthesis

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4
Q

enzyme that does not require a primer

A

DNA dependent RNA polymerase

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5
Q

E.coli polymerase have

A

holoenzyme include sigma factor, require Mg2+ and Zn2+

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6
Q

DNA binding protein that initiates the transcription of bacterial genes is called

A

sigma factor

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7
Q

sigma subunit of RNA polymerase

A

specifies the site for transcription

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8
Q

binding of sigma factor depends on the

A

recognition of the length and spacing of promotor consensus sequence

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9
Q

role of the promotor region of a gene

A

site where RNA polymerase binds to DNA

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10
Q

pribnow box is centered at

A

-10 position

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11
Q

mode of action of rifampicin in E.coli is through

A

initiation of transcription and binding of RNA polymerase to DNA template

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12
Q

TATA box plays a key role in ascending active transcription

A

binding with TATA box binding protein

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13
Q

transcription factors bind to TATA box

A

TFIID

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