EUKARYOTIC TRANSCRIPTION Flashcards

1
Q

regions in mature mRNA is found in the gene for the mRNA

A

start codon, leader sequence, exon

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2
Q

poly A end of eukaryotic mRNA originate from

A

sequential addition of A residues at the 3’ end of primary transcript

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3
Q

eukaryotic mRNA

A
  1. contain a poly A tail upto 250 residue
  2. primary nuclear transcripts are much larger than the cytoplasmic mRNA
  3. a guanosine residue is attached to the 5’ terminus in an unusual 5’-5’ linkage
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4
Q

purpose of storing information in exon separated by intron

A

eukaryotes are able to then use the information in one DNA sequence in alternative ways

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5
Q

spliceosome mediated splicing of GU-AG intron

A

require ATP, involve transesterification reaction, involves the nucleophilic attack on the OH group on the sugar phosphate backbone

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6
Q

snRNA

A

snRNA are strictly localized to the nucleus, most snRNAs are very abundant, some snRNAs can base pair with the intron consensus sequence

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7
Q

introns are released as lariats during splicing of

A

pre- mRNA transcripts and group II intron containing transcripts

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8
Q

alternating splicing means that

A

the same gene can code for several different proteins

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9
Q

splicing event the intermediate do not form the lariat or the branched structure

A

group I intron splicing and hnRNA splicing

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10
Q

introns in tRNA

A

GMP, GDP, GTP

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11
Q

in mammalian cells, RNA editing

A

can change the coding capacity of a gene after transcription, creates a stop codon in the middle of the apo-B- mRNA in intestinal cells but not in liver cells, can change specific nucleotides by deamination

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12
Q

foot printing assay can be used to

A

determine the DNA binding site for the transcription factor

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13
Q

post- transcriptional modifications involves

A

splicing, 5’ capping, 3’ polyadenylation

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