Prokaryotes, Cultures and Gram Stain Flashcards
What is a bacterial colony derived from?
A bacterial colony is derived from a single bacterium that multiplies to form a visible mass.
Must colonies be bacterial?
No, colonies can be formed by other types of organisms, such as fungi.
On what type of media do colonies grow?
Colonies grow on solid media, such as agar plates.
What happens to a broth culture after inoculation and incubation?
The broth becomes cloudy after inoculation and incubation due to bacterial growth.
Is growing a broth culture a way to grow large numbers of bacteria?
Yes, a broth culture is a way to grow large numbers of bacteria.
Are individual colonies observed in a broth culture?
No, individual colonies are not observed in a broth culture.
What do you observe on a smear plate?
You may see individual colonies or colonies that have grown together.
Is preparing a smear plate a way to mass produce bacteria?
No, it is primarily a way to separate individual bacteria from one another.
Why make smear plates instead of broth cultures?
Smear plates allow for isolation of individual colonies, while broth cultures are better for mass production.
Do you observe individual colonies on streak plates?
Yes, individual colonies are observed on streak plates.
Why can’t bacteria digest agar?
This is important for the isolation of individual colonies as it prevents bacteria from spreading throughout the medium.
Why should agar petri plates be stored upside down?
Agar petri plates should be stored upside down to prevent condensation from dripping onto the agar surface.
