prof sample questions - midterm Flashcards
Explain four features that can be used to distinguish members of the Domain Bacteria from the Domain Archaea
a) rRNA sequence
b) Archaeal membrane lipid tails are branched and sometimes linked.
c) Some bacteria can photosynthesize
d) Bacterial cell walls contain some peptidoglycan, Archaea do not.
e) Some Archaea can synthesize methane.
f) Some Archaea can live in extremely hot or saline environments.
Describe two distinct features of viruses that allow them to evolve extremely rapidly
*Extremely rapid reproduction in host cell
*Extremely large number of progeny per reproductive event
*Nucleic acid reassortment can occur when two strains infect the same cell (antigenic shift)
Viruses have been detrimental to human societies in many different ways. Support this
statement using three separate examples
i) Viruses cause many serious human diseases (e.g. COVID 19).
ii) In the history of human colonisation, virus transmission to societies that had not
developed immunity resulted in major local population declines (e.g. Incans,
Mexicans, North American Indigenous peoples)
iii) Viruses could be used a bioterrorism weapon (e.g. anthrax)
iv) Viruses have been implicated in many serious animal and plant diseases that
have affected human food supply
“We now live, as Earth always has, in an Age of Bacteria. These simplest organisms will
dominate our planet (if conditions remain hospitable for life at all) until the sun
explodes.” Stephen J Gould, 1998. This famous biologist considered Prokaryotes as
relatively ‘simple’ organisms compared to Eukaryotes. Make two points in favour of, and two points against this conclusion
Points in favour:
*No nucleus
*No/little membrane compartmentation
*Unicellular (only)
*No cytoskeleton
*No sexual reproduction
*Simple flagella compared to Eukaryotes
Points against:
*Highly diverse metabolic capacities/ecologies
*Extraordinary fundamental (DNA sequence for rRNA) genetic diversity
*Very rapid growth under favourable conditions
*Capacity for very long, highly protected, dormancy
Prokaryotes (and some Eukaryotes) are microscopically small. Outline two positive and two negative implications of being microscopically small
Positives
*Rapid reproduction
*Extensive easy dispersal
Negatives
*Nearby water availability is critical for metabolism
*Large surface area to volume means water medium is very viscous – greatly restricting motility
*Small size means they are readily predated on by larger organisms
Prokaryotes were not only essential to the ancestral origins of the Tree of Life, but also to much of its subsequent development and diversification. Make two points to support the latter notion that prokaryotes have enhanced diversity among the eukaryotes
Endosymbiosis of two prokaryotes gave rise to the first eukaryotes (aerobically respiring,
nucleus-containing organisms), which ultimately diversified into many lineages of heterotrophic eukaryotes
Endosymbiosis of a heterotrophic eukaryote with a cyanobacteria (Prokaryote) gave rise to the first photosynthetic eukaryotes, which ultimately diversified into all the members of the higher
plants (Archaeplastida)
The diverse and unique metabolic capabilities of prokaryotes is critical to the biogeochemical
cycling of many growth-limiting nutrients (N, P, Ca, etc) and therefore to the ongoing
availability of these nutrients to Eukaryotes, thereby providing the resources necessary for their growth and diversification
Prokaryotes such as cyanobacteria were responsible for greatly increasing the concentrations of oxygen in the atmosphere.. thereby providing a critical resource that supports aerobic
respiration in the Eukaryotes
Provide two reasons to explain why prokaryotes are generally only metabolically active when their cells are in contact with external water
Prokaryotic cells are reliant on an external water film around them so that:
a. diffusion can supply nutrients and organic matter
b. diffusion will remove waste excretions
c. water is necessary as a metabolite
Explain what is meant by the term chemoautotroph to describe an organism’s metabolism in terms of its acquisition of energy and carbon, and briefly describe an example of a globally significant biogeochemical reaction that is mediated by a chemoautotroph
A chemoautotroph obtains its energy by chemical oxidation of a reduced compound
such as Fe2+, NH4+, S, CH4, and its carbon by reducing CO2 (or HCO3) gas to an
organic compound such as carbohydrates.
Chemoautotrophs have globally significant and critically important impacts on the
biogeochemical cycling of:
i) iron oxidation (iron-oxidizing bacteria are responsible for some rusting and
rock weathering)
ii) nitrification (nitrifying bacteria convert ammonium in agricultural fertilizer
to nitrate that is particularly prone to leaching into aquatic ecosystems
causing eutrophication)
iii) methanogenesis (some methanogens convert hydrogen to methane -which is a
particularly powerful greenhouse gas)
Describe two lines of evidence supporting the hypothesis that life evolved in deep sea
thermal vents
i) Thermophiles are particularly frequent at the base of the Tree of Life
ii) The reducing conditions in vents are consistent with the biochemical traits of
early life forms (heterotrophic, and anaerobic)
iii) Most of the fundamental housekeeping (cellular maintenance) proteins and
biochemical processes seem to have a heritage reflecting a hydrothermal
environment
iv) UV levels are low in vents relative to the ocean surface meaning nucleic acid
degradation was minimal
v) Strong pH gradient in alkaline-type marine hydrothermal vents may have
favoured the evolution of the ion pumping and membrane system which are
necessities for biochemical energy use. ( Some students may have read the
recommended but not required book by Nick Lane entitled The Vital Question in
which he argues that life probably originated in alkaline-type marine
hydrothermal vents because the strong pH gradient may have favoured the
evolution of the ion pumping and membrane system which are necessities for
biochemical energy use.)
Make four points in favour of the view that viruses are not living organisms
i) Require host cell to replicate.
ii) Do not grow either by size increase or by dividing.
iii) Cannot respond to external stimuli.
iv) Lack cellular structures.
v) Lack capacity to metabolize independently
Elucidating the fundamental biology of viruses has been beneficial to human societies in many different ways. Give an example to demonstrate this point (1 mark), and clearly explain the societal benefit
i) Understanding smallpox and its relation to cowpox led to the development of
vaccines as an immunization preventative medical procedure.
ii) Understanding that many viruses function as bacteriophages has resulted in the
development of very important tools in molecular biology to conduct gene transfers
and cloning.
iii) Understanding the role of the external viral spike proteins that serve as antigens
during human infection has provided the basis for developing mRNA vaccines such
as for COVID-19
The life cycles of all sexually reproducing organisms can be classified into three principal types. Name the generalized life cycle of the type which applies to the Red algae
Alternation of generations
Suggest a reason why these triphasic red algal species may have evolved this capacity for two separate diploid stages
Members of the Red Algae group do not have a flagellum in the gamete/spore
stage, although it seems that their common ancestor with other algal groups did.
Perhaps the flagellum is ineffective for gamete motility in the turbulent coastal
surface water environments in which these organisms grow, and therefore it has
been lost over evolutionary time. The production of multiple successive diploid
forms may be a means to maximize the numbers of sporophytes that will each
contain some cells that undergo meiosisis thereby increasing the numbers of
individual male and female gametophytes and thus gamete production and the
likelihood of compatible mating and successful fertilization.**
Explain two lines of evidence indicating that some Euglenoids gained the capacity to
photosynthesize by secondary endosymbiosis?
Evidence includes the facts that photosynthetic Euglenoids have
chloroplasts that:
a. contain Chlorophyll b
b. are contained by a triple layer of plasma membranes
c. some presumably less advanced (more ancestral) Euglenoids are heterotrophs
Fungi are more important than bacteria and archaea as decomposers of dead plant and
animal organic matter in terrestrial ecosystems. Make three points to support this statement
Fungi can have a hyphal growth form that allows them to grow toward and
within their substrate
Fungi are not as dependent as bacteria on the immediate proximity of water and
nutrients to support metabolism during decomposition because they can
translocate water and nutrients within their hyphae from moist or fertile sources elsewhere, and therefore can decay relatively dry, nutrient-poor substrates.
As a eukaryote, fungi are capable of sexual reproduction meaning that in each successive generation, they can generate relatively high levels of genetic diversity
as compared to bacteria… and therefore they can evolve relatively quickly to
adapt to diverse decomposer niches on land.
Fungi can synthesize specialised wood decay enzymes
Fungi may be better adapted than bacteria to dispersal on land because they can produce large fruiting bodies to release spores into the air and so disperse to other organic matter substrates
Plant colonisation of land occurred long after bacteria evolved, and at about the
same time as the earliest land fungi (~1 bya). Therefore, both fungi and bacteria have had the chance to evolve on organic substrates on land for about the same
time. As a result, the earlier origin of bacteria may not have been any particular
advantage.
Compare and contrast fungal and vascular plant reproduction by describing three
distinctive features of the genetics and lifecycles of the Kingdom Fungi
i) Sexual reproduction is by zygotic meiosis in most fungi whereas vascular plants have an alternation of generations life cycle.
ii) Zygotic (diploid) stage in fungi is unicellular and often represents the
dormant highly protected stage of the life cycle that is favoured when
environmental conditions are not favourable for growth.
iii) Cytoplasmic (plasmogamy) and nuclear (karyogamy) fusion are substantially
separated in time in many fungi (e.g. Ascomycota and Basidiomycota),
meaning there is a distinct and prolonged dikaryotic phase.
iv) Asexual reproduction is extremely common in most fungi, but in a much
smaller proportion of vascular plants.
‘Dry rot’ refers to decomposition of wood in a dry environment by certain fungi. Outline
two distinctive features of these fungi that allow them to achieve this process?
i) Fungi have the capacity to translocate water and nutrients along the mycelium from hyphae that are in contact with moist areas elsewhere to other hyphae that are
actively decomposing completely dry wood
ii) Certain dry rot fungi can synthesize substantial liquid water as a byproduct of
glycolysis
iii) Dry rot fungi can synthesize highly specialized wood-decaying enzymes
Describe the structure of a crustose lichen in cross-section by explaining each of the four
layers in sequence from the top surface to the base
Sandwich structure with alga enclosed beneath a thin upper relatively dense layer of
fungal tissue, with a thicker more ‘spongey/open/porous fungal layer below the algal layer, and then a thin fungal layer at the base
Explain the function of the fungal inner layer
The inner layer of low density fungal tissue provides excellent water uptake and storage.
Indicate how lichens acquire their supplies of carbon and sulfur and which symbiont participant is involved in each case
Alga fixes CO2 during photosynthesis, some of which is used for its own metabolism and
some of which is translocated to the fungus.
Sulfur is generally acquired from atmospheric deposition of particles and in soluble form
in rainfall onto the uppermost surface fungal layer
Diversity begets diversity. Explain this central concept of the course
The evolution of diverse traits among species enhances the potential for
subsequent evolution of further diversity in traits, and ultimately often leads to
increased numbers of species
Trillium species, Daffodils and Lilies are all members of the Monocot plant group.
Describe two common structural features of the flowers in this group
Radial symmetry (generally)
Floral parts in threes
Embryo that produces single cotyledon
Describe a distinctive characteristic feature of the emerging germinating embryo in monocot plant group
All monocots have a single cotyledon leaf.. and so on germination this single leaf
is the first structure to emerge. (Technical term for first monocot leaf is
coleoptile. By contrast, the the germinating embryo of the Eudicota all have two
emerging leaves (cotyledons).)
Describe the typical mature leaf shape of the monocot plant group
Leaves are usually long and often narrow, terminating in a single-point (i.e.
without indentation (grass-like as compared with a Eudicot maple leaf for example). (Leaf veins are usually parallel)