Processing & Evaluation of Milk Quality Flashcards

1
Q

Introduction to milk

A

❑ Milk is a type of emulsion that consists of 3.9% fat, 8.8% Non-Fat-Solids, and 87.3% of water.

❑ Cow milk contains low fat content (3.66%).

❑ Buffalo milk contains high fat content (7.44%).

❑ Milk is highly perishable commodity and likely to be
spoiled during transportation due to it is an excellent medium for the growth of microorganisms, particularly bacterial pathogens that can cause spoilage and disease in consumers

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2
Q

Classification of milk

A

1) Solution
2) Dispersion
3) Emulsion

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3
Q

Solution (milk):

A

Contains the sugar lactose, the water-soluble vitamins thiamin and riboflavin, and many mineral salts such as calcium, phosphate, citrates, and the minerals chloride, magnesium, potassium, and sodium

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4
Q

Colloidal dispersion (Milk):

A

A colloid is any material in which tiny particles of one substance are spread through a larger volume of another substance. E.g., casein and whey proteins and milkfat globules dispersed in watery liquid

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5
Q

Emulsion (Milk):

A

Fat globules suspended in the aqueous phase (serum) of
milk.

The fat globules are surrounded by a complex membrane, the milk fat globule membrane, which contains mainly protein and phospholipids (and a few carbohydrate side chains at the outer surface).

This membrane prevents coalescence of the fat droplets.

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6
Q

MILK,RAW MILK OR FRESH MILK (FOOD ACT 1983 & REGULATIONS 1985)

A

(1) In these Regulations,“milk”,“raw milk” or “fresh milk” means the normal, clean,fresh mammary secretion of healthy cow,buffalo, goat, or sheep that is properly fed and kept,excluding that obtained during the four days immediately following calving.

(2) Milk

(a)Shall contain not less than -
(i) 3.25 per cent of milk fat;and
(ii) 8.5 per cent of non-fat milk solids; and

(b) Shall not contain any –
(i) added water;
(ii) permitted food additive;
(iii) other added substance;or
(iv) trace of antibiotic substance

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7
Q

Milk Processing - Pasteurization

A

• Pasteurization minimizes the likelihood of disease and extends the storage life of milk.

• Pasteurization temperatures do not change milk components.

• Vitamin destruction and protein denaturation are minimal, and the result is that milk is made safe for consumption.

• The shelf-life of pasteurized milk held under 7 ℃ - 12 to 21 days (if unopened)

• Actually, Coxiella burnetii, which causes Q fever requires an even higher temperature for destruction; thus, the required 63 ℃ for pasteurization.

• The high pasteurization temperature, followed by rapid cooling, controls nonpathogenic growth.

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8
Q

LTLT

A

63 ℃ for 30 or more minutes; the batch or holding method and is considered low-temperature longer time (LTLT) pasteurization.

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9
Q

HTST

A

72 ℃ for 15 seconds; the flash method and is the High-Temperature Short-Time (HTST) method of pasteurization.

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10
Q

Milk Processing – Sterilization (Ultra-pasteurization)

A

• Sterilization is pasteurization that occurs at higher temperatures with a different time:

i. 138–150 ℃ for 2–6 seconds
ii. “Ultrapasteurized when used to describe a dairy ingredient means that such ingredient shall have been thermally processed at or above 138 ℃ for at least 2 seconds.”

• Ultra high temperatures (UHT) processing - It does not allow spoilage or pathogenic bacteria to enter the milk.

• If packaging too is sterilized, the package is referred to as being “aseptically packaged” and hermitically sealed.

• Milk treated in this manner may be safely stored up to 3 months or longer (if unopened)

• UHT milk will often have cooked flavor.

• Can keep at room temperature

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11
Q

Homogenization

A

• The primary function of homogenization is to prevent
creaming, or the rising of fat to the top of the container of milk (whole or some low-fat milk).

• The result is that milk maintains a more uniform composition with improved body and texture, a whiter appearance, richer flavor, and more digestible curd.

• The homogenization reduces fat globule size from an average of 3.5 µm in diameter to below 1 µm

• Preventing globules from reuniting or coalescing, and the fat remains homogeneously distributed throughout milk.

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12
Q

Fortification

A

• The addition of fat-soluble vitamins A and D to whole milk.

• Low-fat milk, nonfat milk, and low-fat chocolate milk must be fortified (usually before pasteurization) to carry 2000 international units (IU) vitamin A.

• Vitamin D to reach levels of 400 IU’s is optional but routinely practiced.

• Nonfat milk solids (MSNF) may be added to milk to low-fat milk - and it will state “protein fortified” or “fortified with protein” on the label

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13
Q

Evaporated and Concentrated Milks

A

• Evaporated milk (unsweetened condensed milk) is
concentrated through the process of evaporation (at 50–55 ℃) in a vacuum chamber.

• Either whole or nonfat milk with 60% of the water removed is then homogenized, fortified with vitamins A and D, canned, and sterilized in the can (115–118 ℃) in a pressure canner.

• Whole evaporated milk must contain not less than 25% total milk solids and not less than 7.5% milkfat.

• Evaporated nonfat milk must contain not less than 20% milk solids and no more than 0.5% milkfat.

• It must be fortified with 125 and 25 IU of vitamins A and D, respectively.

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14
Q

Sweetened Condensed Milk

A

• Sweetened condensed milk is concentrated whole or nonfat milk with approximately 60% of the water removed and sugar levels of 40–45% in the finished product.

• There is a calorie difference in this milk processing, as whole sweetened condensed milk contains no less than 8% milkfat and 28% total milk solids and nonfat contains no
more than 0.5% milkfat and 24% total milk solids.

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15
Q

Dried Milk

A

• Dried milk powder may be processed from either pasteurized whole or more commonly from nonfat milk.

• One method of drying involves spray drying.

• Milk is first condensed by removing two-thirds of the water and to dry to less than 5% moisture levels.

• Most nonfat dried milk is fortified with vitamins A and D.

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16
Q

Cultured milk/fermentation

A

• Cultured products are fermented by the addition of bacterial cultures, such as lactobacilli and streptococci, to fluid dairy products.

• Lactose is fermented to lactic acid, creating a low pH in the process, which
(1) controls both spoilage and pathogenic bacterial growth,
(2) causes the casein to coagulate.

• Because cultured and acidified products contain different amounts of lactic acid, they differ in flavor.

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17
Q

Buttermilk

A

• Traditionally, buttermilk was the liquid that remained
when cream was churned to form butter.
• It is more correctly named “cultured low-fat milk” or
“cultured nonfat milk.”

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18
Q

Process of Buttermilk

A

• The starting ingredient for buttermilk is skim or low-fat
milk. The milk is pasteurized at 82 to 88 °C for 30 minutes, or at 90 °C for two to three minutes.

• The milk is then cooled to 22 °C , and starter cultures
of desirable bacteria, such as Streptococcus lactis, S. cremoris, Leuconostoc citrovorum, and L. dextranicum, are added to develop buttermilk’s acidity and unique flavor.

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19
Q

Sour cream

A

• Traditionally, sour cream was made from heavy (whipping) cream that was soured.

• Today, it is made from pasteurized, homogenized, fresh, light cream (approximately 18% fat).

• While inoculation and fermentation steps are similar to buttermilk production, fermentation is shortened.

• S. lactis and Leuconostoc bacteria may be added for
flavor, and stabilizers such as gelatin or gums may be
present.

• Nonfat milk solids may be added to thicken the cream.

• A bitter taste in sour cream that is stored more than 3–4 weeks may form due to proteolytic bacterial enzyme activity.

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20
Q

Yogurt

A

• Yogurt is the food produced by culturing one or more of the pasteurized fluid dairy ingredients such as cream, milk, partially skimmed milk, or skim milk (used alone or in combination depending on the desired fat content) with a
bacteria culture.

• The milk is both pasteurized and homogenized before the addition of a starter that contains the lactic-acid producing bacteria, L. bulgaricus and S. thermophilus.

21
Q

Yogurt’s procedure

A

• Yogurt may be made using whole, low-fat, or skim milk.

• The formulation may include nonfat dry milk (NFDM) or condensed skim milk to boost its solids.

• It contains not less than 8.5% MSNF and not less than 3.25% milkfat.

• Or it may be prepared to be a reduced or low-fat yogurt and have levels of 0.5–2.0% milkfat or less.

• Microorganisms in yogurt exist in a “friendly” form, known as probiotic flora.

22
Q

Acidophilus milk

A

• Acidophilus milk is a cultured product made from
pasteurized low-fat, nonfat, or whole milk.

• Lactobacillus acidophilus is added and incubated at 37 ℃.

• A variation of this is sweet acidophilus milk. This sweet version has culture added.

• It is thought to be therapeutic without the characteristic high acidity and flavor.

• Acidophilus produces the enzyme lactase and helps correct the symptoms of lactose intolerance.

23
Q

Kefir

A

• It contains the bacteria Lactobacillus caucasius and the yeasts Saccharomyces kefir and Torula kefir.

• As well, it is slightly bubbly due to the fermentation process, and it contains approximately 1% alcohol.

• Other specialty types of milk include low-sodium, lactose-reduced milk, calcium-fortified, as well as flavored milks and shakes.

24
Q

Butter

A

• Today, there are various blends of butter and margarine in the market.

• The fat composition and taste differ from the original.

• Margarine, or oleomargarine, is the food in “plastic cream” form or liquid emulsion containing not less than 80% fat.

• It may be unsalted or lactose-free.

• It contains vitamin A and may contain vitamin D.

• Sweet cream butter is made by the addition of S. diacetyllactis, which ferments the citrate in milk to acetaldehyde, acetic acid, and diacetyl, the last being the major flavor compound of butter.

25
Q

Butter process

A

• Butter is a concentrated form of fluid milk produced through churning of pasteurized cream.

• Churning involves agitation that breaks fat globule membranes, so the emulsion breaks, fat coalesces, and water (buttermilk) escapes.

• Emulsions may be of two types. The original 20/80 oil-in-water type of emulsion of milk becomes a 20/80 water-in-oil emulsion.

• Milk is churned to form butter and the watery buttermilk.

• Butter may have a yellow color due to the fat-soluble animal pigment, carotene, or an additive.

26
Q

Cream

A

• Cream is the high-fat component separated from whole milk as a result of the creaming process.

• It has a higher proportion of fat droplets to milk than regular fluid milk; and according to federal standards of identity, cream must contain 18% milkfat or more.

• Due to this high fat content of cream compared to milk, some yellow, fat-soluble pigments may be apparent

27
Q

Various liquid creams available for use in foods include the following:

A
  1. Light (coffee) cream: 18–30% butterfat.
  2. Light whipping cream: 30–36% butterfat.
  3. Heavy cream: minimum 36% butterfat.
  4. “Half-and-half” cream diluted with nonfat milk: butterfat.
  5. Whipping cream packaged under pressure in aerosol cans; may be contain various levels of fat, sugar, flavoring, emulsifiers, and a stabilizer.
28
Q

Cheese

A

A product made from curd obtained from the whole, partly skimmed, or skimmed milk of cows, or from milk of other
animals, with or without added cream, by coagulating with rennet, lactic acid, or other suitable enzyme or acid, and
with or without further treatment of the separated curd by heat or pressure, or by means of ripening ferments, special molds, or seasoning”

29
Q

Cheese process

A

• Cheese is a concentrated form of milk that contains casein, various percentages of fat, primarily saturated fat, mineral salts, and a small portion of milk serum (whey proteins, lactose, and water-soluble vitamins).

• It is the curd that forms as a result of casein coagulation by the enzyme rennin (also known as chymosin) or lactic acid.

• Rennet is the name given to any enzymatic preparation that clots milk

30
Q

Hard cheese

A

Hard cheese contains 30–40% water. It has very tiny fat globules and is a near-perfect emulsion.

31
Q

Soft cheese

A

Soft cheese contains 40–75% water and has large fat globules. It is only slightly emulsified.

32
Q

Ripening

A

• Ripening may require 2–12 months. In that time, the;

i. carbohydrate lactose is fermented by lactase to lactic acid,
ii. fat is hydrolyzed by lipase, and
iii. protein undergoes mild proteolysis to amino acids by rennin.

• Ripening refers to the chemical and physical changes that occur in the cheese in the time between curd precipitation and satisfactory completion of texture, flavor, aroma, and color development.

• Ripening modifies the characteristics mentioned, as well as continuing to ferment residual lactose.

• It may be due to bacteria, bacterial enzymes (chiefly rennin), or the fungus mold and yeast.

33
Q

Ice Cream

A

• It is a food produced by freezing, while stirring a pasteurized mix containing dairy product.

• The mix consists of one or more dairy ingredients such as cream, milk, skim milk, sweet cream buttermilk or sweetened condensed milk, and optional caseinates.

• Typically, sugar (sucrose, dextrose, which flavors and depresses the freezing point), cookies, eggs, fruit, nuts, and other ingredients such as coloring or flavoring agents, emulsifiers [such as egg yolks, polysorbate 80 (a sorbitol ester consisting of a glucose molecule bound to the fatty acid; oleic acid), or mono and diglycerides], stabilizers (gelatin, vegetable gum), and water are added

34
Q

Process of ice cream

A

• The ice cream mix is subject to pasteurization, homogenization, holding (for aging), and quick freezing.

• Slow freezing creates larger ice crystals.

• Air is naturally incorporated into an ice cream mixture by agitation.

35
Q

Overrun

A

• The increase in volume due to air is overrun

• Overrun is the term for the percent of the expansion of ice cream achieved from the amount of air incorporated into the product during the freezing process.

• An overrun of 50% means that it has expanded 50%

%Overrun = (Volume of ice cream−Volume of mix) ×100%
Volume of mix

36
Q

QUALITY ASSESSMENT OF RAW MILK

A
  1. Organoleptic tests
  2. Sediment test
  3. Clot on boiling (C.O.B) test
  4. The alcohol test
  5. Alcohol-alizarin test
37
Q

PLATFORM TEST - ORGANOLEPTIC TESTS

A

 It refers to the test, which are performed to check the quality of incoming milk on the receiving platform so as to make quick decision regarding it’s acceptance or rejection.

 Some of the platform tests are:

❖ Smell (Odor): This is an excellent indication of organoleptic quality of milk. An experienced milk grader with ‘trained nose’ usually relies to a great extent in the acceptance/rejection of the intake milk on odor test alone.

❖ Appearance: Milk should be normal in color (yellowish creamy – cow milk) free from churned fat globule and
free from extraneous matter.

❖ Temperature: A temperature 5 °C or below is satisfactory.

38
Q

PLATFORM TEST - SEDIMENT TEST

A

 This test is to know the cleanliness/extent of visible dirt present in the milk as a mark of clean milk production.

39
Q

SEDIMENT TEST Process

A

❖ Take a milk sample from well stirred can of milk with the help of sampling dipper.

❖ Filter the milk through properly adjusted firm link disc held in the sediment tester,so that a filtration area of 28 mm in diameter is exposed.

❖ Remove the cotton disc from sediment tester after filtration.

❖ A good quality milk gives no visible dirt whereas poor quality milk shows dark or blackish deposits on the filter pad.

40
Q

PLATFORM TEST - CLOT ON BOILING (C.O.B)TEST

A

 The test is quick and simple.

 It is to determine the stability of milk for heat processing (i.e.,pasteurization).

 It is one of the old tests for too acid milk (pH<5.8) or abnormal milk (e.g.,colostral or mastitis milk).

 If a milk sample fails in the test,the milk must contain many acid or rennet producing microorganisms, or the milk has an abnormal high percentage of proteins like colostrum milk.

 Such milk cannot stand the heat treatment in milk processing and must therefore be rejected.

41
Q

PLATFORM TEST - THE ALCOHOL TEST

A

 The test is quick and simple.

 Suitable for milk that requires high heat processing such as condensing and UHT processing,which it has to be highly heat stable.

 This test is to detect abnormal milk such as colostrum or mastitis milk.

 It is based on instability of the proteins when the levels of acid and/or rennet are increased upon added with alcohol.

 Also, increased levels of albumen (i.e, colostrum milk), high acidity, or salt concentrates (mastitis) results in a positive test.

42
Q

PLATFORM TEST - ALCOHOL-ALIZARINTEST

A

 Alcohol-alizarin test illustrates the suitability of milk for high heat treatment and also gives an idea about its acidity.

 To 5 mL milk in a test tube is added an equal amount of alcohol-alizarin solution (0.2%).

 The contents are mixed and observed for formation of flakes and colour, and the observations matched with the
chart for alcohol-alizarin test.

43
Q

CHEMICAL QUALITY OF MILK (ACIDITY TEST)

A

 In the acidity test, the acid is neutralized with 0.1 N Sodium hydroxide, and the amount of alkaline is measured.

 From this,the percentage of lactic acid can be calculated.

 Titratable acidity (as lactic acid per 100 ml of milk) = 0.9VN
 V= Volume of NaOH required for titration of 10 ml of milk sample

 N= exact normality of the standard sodium hydroxide solution

 The normal range: 0.1- 0.17% lactic acid; acidity >0.17%

 Values higher than this signifies developed acidity due to the action of bacteria on milk sugar.

44
Q

DETECTION OF PRESERVATIVES, NEUTRALIZERS, AND ADULTERANTS

A

 The addition of any kind of preservative is not legally permitted.

 However, suppliers may add various kinds of preservatives (e.g., boric acid, formalin/formaldehyde, hydrogen peroxide etc.) to milk in order to increase its shelf life.

 Several neutralizing agents (e.g., sodium/potassium hydroxide,sodium carbonate/bicarbonate etc.) are added tomask the developed acidity in milk.

 Various adulterants (e.g., cane sugar, starch, urea, maltodextrin etc.), which are not native to milk, are added to increase or maintain the prescribed levels of solid non fat (SNF) and total solid (TS) of milk.

45
Q

DETECTION OF COMMON PRESERVATIVES
(BORICACIDAND BORATES)

A

▪ Adding boric acid and borax to raw milk can prevent it from souring and curdling by reducing its acidity, thereby extending its shelf life.

 When a strip of turmeric paper is dipped into adulterated milk sample, it turns into red color indicating the presence of boric acid and its salt.

46
Q

DETECTION OF COMMON PRESERVATIVES
(FORMALIN OR FORMALDEHYDE BY LECHTEST)

A

 To 5 mL of milk in a test tube is added equal volume of concentrated hydrochloric acid containing 1 mL of 10% ferric chloride solution.

 The contents are heated over a flame for 5 min, and the tube rotated or shaken to break the curd.

 Development of violet color indicates the presence of formaldehyde.

47
Q

DETECTION OF COMMON PRESERVATIVES
(HYDROGEN PEROXIDE)

A

 Hydrogen peroxide acts as an antimicrobial agent thereby, checking the development of acidity.

 Addition of 2 drops of 2% freshly prepared aqueous solution of paraphenyl diamine hydrochloride to the adulterated milk sample gives intense blue color, thus indicating the presence of hydrogen peroxide.

48
Q

DETECTION OF NEUTRALIZER

A

 Chemicals such as carbonates or bicarbonates are added to milk in order to alter the developed acidity.

 An equal quantities of the milk sample and ethyl alcohol are mixed. Then, few drops of aqueous rosalic acid solution were added to detect their presence,

 Presence of rose red color indicates addition of sodium hydroxide, potassium hydroxide, and/or calcium hydroxide.

 Presence of pink color points to the addition of sodium bicarbonate, potassium carbonate, or calcium carbonate.

49
Q

DETECTION OF COMMONADULTERANTS
STARCH (IODINETEST)

A

 Starch is one such component that is added to adulterate
milk.

 The test to detect starch in milk uses iodine solution (1%)

 3 mL of well-mixed milk is boiled over a Bunsen burner.

 After cooling, one drop of 1% iodine solution is added
and mixed well.

 The appearance of an intense blue or bluish violet color due to the formation of starch-Iodo complex, in the presence of starch.