Process of PCR Flashcards
0
Q
Step One
A
Denature the DNA strand
- sample is heated to 94-96°C
1
Q
What is PCR?
A
A process used by scientists that copies small segments of DNA very quickly.
2
Q
Step Two
A
Primers
- temperature is lowered to 50-65°C
- left and right primers base pair with complementary sequences
3
Q
Step Three
A
Synthesis
- temperature is raised to 72°C
- Taq Polymerase attached at each priming site
- synthesized new DNA strand
4
Q
What next?
A
Process continues for however many strands they want replicated.
5
Q
What is it used for?
A
- replicate DNA for lab procedures
- produces millions of copies
• no bacteria needed
