Procedure of Hematocrit

Question 1

1. Fill ___ plain capillary tubes approximately _____ full with blood anticoagulated with ____ or ____.

Answer 1

1. 2
2. 3 quarters
3. EDTA or heparin

Question 2

2. ____ the end of the tube w/ the ____ using ____.

Answer 2

1. Seal
2. colored ring
3. non-absorbent clay

Question 3

3. Hold the filled tube ____ & seal by placing the ____ end into the tray w/ sealing compound at a ____.

Answer 3

1. horizontally
2. dry
3. 90 degree angle

Question 4

4. Rotate the tube slightly and remove it from tray. The ____ should be at least 4mm long.

Answer 4

Plug

Question 5

5. Balance the tubes in a micro-Hct centrifuge with the clay ends facing the ____ away from the ____, touching the ____.

Answer 5

1. outside
2. center
3. rubber gasket

Question 6

6. Tighten the head cover on the centrifuge & close the top. Centrifuge the tubes at ____ to ____ for the time that has been determined to obtain ____ of RBCs.

Answer 6

1. 10 000g
2. 15 000g
3. maximum packing

Question 7

7. Determine the hct by using a ____. Read the level of ____; do not include ____ when taking the reading.

Answer 7

1. micro-Hct reading device
2. RBC packing
3. buffy coat

Question 8

8. The values of the ____ should agree within 1%.

Answer 8

duplicate Hcts