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Hematology > Cyanmethemoglobin > Flashcards

Cyanmethemoglobin Flashcards

1
Q

Methemoglobin + potassium cyanide

A

Cyanmethemoglobin

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2
Q

It is the reference method for hemoglobin assay & the best and most widely used

A

Cyanmethemoglobin

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3
Q

What is the referene method for hemoglobinemetry

A

Cyanmethemoglobin

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4
Q

What reagent does cyanmethemoglobin use?

A

Drabkins reagent

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5
Q

What comprises Drabkin’s reagent?

A
  1. potassium cyanide
  2. potassium ferricyanide
  3. sodium carbonate
  4. surfactant
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6
Q

Other term for Cyanmethemoglobin method?

A

Hemiglobincyanide method

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7
Q

T/F: Does extremely ↑ WBC count & platelet count falsely elevate the values?

A

TRUE

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8
Q

Methemoglobin –> cyanmethemoglobin

A

Potassium cyanide (KCN)

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9
Q

It oxidizes Hb –> methemoglobin

A

Potassium ferricyanide (K3Fe(CN)6)

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10
Q

It determines hemoglobin

A

Cyanmethemoglobin method

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11
Q

An ionic surfactant that reduces environmental cyanide

A

Sodium lauryl sulfate (SLS)

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12
Q

Hb –> SLS-methemoglobin

A

SLS

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13
Q

T/F: SLS does NOT generate toxic wastes

A

TRUE

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14
Q

It cannot be converted to cyanmethemoglobin

A

Sulfehemoglobin

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15
Q

T/F: Sulfehemoglobin is used in cyanmethemoglobin method

A

FALSE; it is NOT used

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16
Q

T/F: Drabkin reagent is a strong solution.

A

FALSE; it is a WEAK solution

17
Q

At what wavelength does the absorbance or color intensity of the solution is measured

A

540 nm

18
Q

T/F: Free hb combines w/ potassium ferricyanide

A

TRUE

19
Q

Reagent that converts Hb = ferrous –> ferric

A

Cyanmethemoglobin reagent

20
Q

T/F: Cyanmethemoglobin color intensity is proportional to hb concentration.

A

TRUE

21
Q

T/F: Cyanmethemoglobin method is not approved by CLSI

A

FALSE; it is approved to be the reference method for Hb determination

22
Q

Standard used for cyanmethemoglobin method

A

80mg/dL

23
Q

Anticoagulant used

A

Heparin or EDTA

24
Q

To allow full conversion of hb to cyanmeth, how many minutes should the specimen stand at room temp?

A

10 minutes

25
Q

T/F: Cyanmeth is not sensitive to light

A

FALSE; it is sensitive to light and must be stored in a brown and dark place

26
Q

Interval of ↑ WBC count

A

> 20x10^9 /L

27
Q

Interval of ↑ platelet count

A

> 700x10^9 /L

28
Q

Handheld system in which hb –> azidemethemoglobin

A

HemoCue

29
Q

Wavelengths of Hemocue

A

570 nm & 880nm

Hematology (4 decks)

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