Cyanmethemoglobin Flashcards
Methemoglobin + potassium cyanide
Cyanmethemoglobin
It is the reference method for hemoglobin assay & the best and most widely used
Cyanmethemoglobin
What is the referene method for hemoglobinemetry
Cyanmethemoglobin
What reagent does cyanmethemoglobin use?
Drabkins reagent
What comprises Drabkin’s reagent?
- potassium cyanide
- potassium ferricyanide
- sodium carbonate
- surfactant
Other term for Cyanmethemoglobin method?
Hemiglobincyanide method
T/F: Does extremely ↑ WBC count & platelet count falsely elevate the values?
TRUE
Methemoglobin –> cyanmethemoglobin
Potassium cyanide (KCN)
It oxidizes Hb –> methemoglobin
Potassium ferricyanide (K3Fe(CN)6)
It determines hemoglobin
Cyanmethemoglobin method
An ionic surfactant that reduces environmental cyanide
Sodium lauryl sulfate (SLS)
Hb –> SLS-methemoglobin
SLS
T/F: SLS does NOT generate toxic wastes
TRUE
It cannot be converted to cyanmethemoglobin
Sulfehemoglobin
T/F: Sulfehemoglobin is used in cyanmethemoglobin method
FALSE; it is NOT used
T/F: Drabkin reagent is a strong solution.
FALSE; it is a WEAK solution
At what wavelength does the absorbance or color intensity of the solution is measured
540 nm
T/F: Free hb combines w/ potassium ferricyanide
TRUE
Reagent that converts Hb = ferrous –> ferric
Cyanmethemoglobin reagent
T/F: Cyanmethemoglobin color intensity is proportional to hb concentration.
TRUE
T/F: Cyanmethemoglobin method is not approved by CLSI
FALSE; it is approved to be the reference method for Hb determination
Standard used for cyanmethemoglobin method
80mg/dL
Anticoagulant used
Heparin or EDTA
To allow full conversion of hb to cyanmeth, how many minutes should the specimen stand at room temp?
10 minutes
T/F: Cyanmeth is not sensitive to light
FALSE; it is sensitive to light and must be stored in a brown and dark place
Interval of ↑ WBC count
> 20x10^9 /L
Interval of ↑ platelet count
> 700x10^9 /L
Handheld system in which hb –> azidemethemoglobin
HemoCue
Wavelengths of Hemocue
570 nm & 880nm
