Principles Of Genetics Flashcards
1
Q
Pol I
A
Transcribes rRNA genes
2
Q
Pol II
A
Transcribes mRNA genes
3
Q
Pol III
A
Transcribes tRNA
4
Q
Prokaryotic gene expression
A
- no RNA splicing
- single RNA polymerase
- mRNAs commonly polycistronic
- transcription and translation occur simultaneously
5
Q
Eukaryotic gene expression
A
- genes monocistronic
- 3 distinct RNA polymerases
- introns and exons
- DNA packaged into chromatin
6
Q
Introns
A
Removed from mRNA
7
Q
Exon
A
Sections kept in mRNA
8
Q
PCR
What do you use?
Benefits
Limitations
A
Can use either DNA or RNA (if using RNA FIRST STEO IS TO USE A REVERSE TRANSCRIOTASR TI convert to DNA)
Quick
Inexpensive
Only need a little DNA - can literally start with a single cell
Can be quantitative- meaning you can look at relative amounts
Limitations:
Can only do a region of DNA up to about 1000 base pairs
Need to know gene sequence to label a mutation
9
Q
DNA sequencing
A
Can sequence up to 500 based
Can detect single base changes or small insertions or deletions
Starts with the PCR product
