Principles Of Diagnosis Of Infectious Disease 1

Question 1

Why we need to diagnose?

Answer 1

1. Treat 2. Prevent

Question 2

what do Anaerobes need to be transported?

Answer 2

Thioglycollate

Question 3

Giemsa stain

Answer 3

Detects parasites in blood &some intracellular bacteria

Question 4

Dark field microscopy

Wet mounts

Answer 4

Trichomonas vaginalis
Treponema pallidum (spirochetes) syphilis
Useful for observing motility and morphology

Question 5

Staphylococcus aureus

Answer 5

Positive
Skin abscess
Cocci

Question 6

Bacillus anthracis

Answer 6

+ rods

Question 7

Streptococcus pneumoniae

Answer 7

+ diplococci

Question 8

Neisseria gonorrhoeae

Answer 8

• diplococci

Question 9

Pus cell

Answer 9

-

Question 10

Neisseria meningitidis

Answer 10

• diplococci

Question 11

E. coli

Answer 11

• rods

Question 12

Mycobacterium tuberculosis

Answer 12

Ziehl neelsen ( acid fast stain)

Question 13

India ink

Answer 13

Rapid detection of C. Neoformans in clinical specimens
Ink darkens the background not the cell
-

Question 14

Cryptococcus neoformans

Answer 14

Polysaccharide capsule around yeast cell

India ink

Question 15

Auramjne rhodamine stain

Answer 15

( acid fast bacilli)

Fluroscent dye

Question 16

Chlamydia trachomatis

Answer 16

Fluroscent

Question 17

Time taken to culture

1. Virus
2. Bacteria
3. Fastidious organisms

Answer 17

1. Days - weeks
2. Hours - days
3. (Require specific growth factors to grow)
   - haemophilus influenza
   - streptococcus pneumoniae
   - N. Meningitidis & N. Gonorrhoeae