Bacterial Identification

Question 1

Proteus mirabilis

Proteus vulgaris

Answer 1

Swarming motility

Question 2

Pseudomonas aeruginosa

Answer 2

Pigmentation

Question 3

KLkebsiella pneumoniae

Answer 3

Mucoid colony

Question 4

Streptococci species

Answer 4

Hemolysis in blood ager

Question 5

Alpha hemolysis

Answer 5

Streptococcus pneumoniae

Partial lysis

Question 6

Beta hemolysis

Answer 6

Streptococcus pyogones

Complete lysis

Question 7

Gamma hemolysis

Answer 7

Enterococcus faecalis

No lysis

Question 8

Specific enzyme catalase test

Answer 8

Staphylococci (catalase +)

Streptococci (catalase -)

Question 9

Specific enzyme Oxidase test

Answer 9

Pseudomonas aeruginosa (oxidase +)
E. coli (-)
Detect presence of cytochrome oxidase enzyme

Question 10

Coagulate test

Answer 10

Staphylococcus aureus (coagulase positive)
Staphylococcus epidermidis and staphylococcus saprophyticus (-)

Question 11

Optochin

Answer 11

Used to differentiate between streptococcus pneumoniae from other alpha hemolytic streptococci

S. Pneumoniae (sensitive)
Viridans streptococcus (resistant)

Question 12

Bacitracin

Answer 12

Used to differentiate s. Pyogenes

S pyogenes (sensitive)

Question 13

Novobiocin

Answer 13

Used to differentiate staphylococcus saprophyticus from another coagulase-negative staphylococcus epidermidis

Staphylococcus saprophyticus(resistant)
Staphylococcus epidermidis (sensitive)