Principles

Question 1

What is bichromatic blanking

Answer 1

Measuring the absorbance of a blank
Measuring absorbance at 2 wavelengths to provide a blank reference point

Question 2

What light source is used in a spectrophotometer

Answer 2

Continuous spectrum

Question 3

Colorimetric assay

Answer 3

The use of a spectrophotometer to determine the concentration of a coloured compound in solute

Read absorbance of sample

Beer Lamberts law

Question 4

Nephelometric

Answer 4

Used to detect the concentration of proteins in serum
Based on the concept that particles in solution will scatter light passing through the solution rather than absorbing the light

Question 5

Photometric

Answer 5

To measure the intensity of light transmitted through a sample by converting light into electricity

Only uses light only in the visible range

Question 6

Turbidimetric

Answer 6

The measurement of turbidity by quanitfying the degree of ‘attenuation’ of a beam of light of known initial intensity

Question 7

End point

Answer 7

An enzyme assay that is run for a predetermined amount of time

The amount of substrate consumed and/or the quantity of product formed is measured over the coruse of the reaction

Used to determine the rate of a reaction

Question 8

Kinetic

Answer 8

The progress of the reaction is continuously measured as substrates are converted into products

Measure at multiple time intervals to produce a graph of absorbance vs time