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Plating Media for Routine Bacteriology > Primary Purpose > Flashcards

Primary Purpose Flashcards

1
Q

Differential isolation and presumptive identification of group D streptococci and enterococci

A

Bile esculin agar (BEA)

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2
Q

Selective and differential for cultivation of vancomycin-resistant enterococci from clinical and surveillance specimens

A

Bile esculin azide agar with vancomycin

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3
Q

Cultivation of nonfastidious microorgan-isms. determination of hemolvtic reactions

A

Blood agar (BA)

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4
Q

Isolation of Bordetella pertussis and
Bordetella parapertussis

A

Bordet-Gengou Agar

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5
Q

Cultivation of fastidious organisms

A

Brain-heart infusion agar
or broth

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6
Q

Enrichment for Legionella spp.
Supports the growth of Francisella and
Nocardia spp.

A

Buffered charcoal-yeast extract agar (BCYE

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7
Q

Enrichment and selection for
Legionella spp.

A

Buffered charcoal-yeast extract (BCYE) agar with antibiotics

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8
Q

For recovery of B. cepacia from cystic
fibrosis

A

Burkholderia cepacia selective agar

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9
Q

Selective for Campylobacter spp.

A

Campy-blood Agar

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10
Q

Selective holding medium for recovery of Campvlobacter spp. Incubated at 4°C for cold-enrichment

A

Campylobacter thioglycollate broth

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11
Q

Improved growth of obligate, slow-
growing anaerobes

A

CDC* anaerobe 5% sheep blood agar

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12
Q

Selective medium for isolation of
Campylobacter spp.

A

Cefoperazone, vancomy-cin, amphotericin (CVA) medium

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13
Q

Selective for Yersinia Spp.: may be useful for isolation of Aeromonas spp.

A

Cefsulodin-irgasan-novobiocin (CIN) agar

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14
Q

Cultivation of fastidious microorganisms such as Haemophilus spp., Brucella spp. and pathogenic Neisseria spp.

A

Chocolate agar

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15
Q

Designed to optimize growth and differentiate a specific type of organism; routinely used in the identification of yeasts, methicillin-resistant Staphylococcus aureus (MRSA), and a variety of other organisms

A

Chromogenic media

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16
Q

Selective isolation of gram-positive coccI

A

Columbia colistin-nalidixic acid (CNA)
agar

17
Q

Isolation of Corynebacterium diphtheria

A

Cystine-tellurite blood agar

18
Q

Isolation and differentiation of lactose-fermenting and non-lactose-fermenting enteric bacilli

A

Eosin methylene blue
(EMB) agar (Levine)

19
Q

Selective (enrichment) liquid medium for enteric pathogens

A

Gram-negative broth (GN)

20
Q

Differential, selective medium for the isolation and differentiation of Salmonella and Shigella spp. from other gram-negative enteric bacilli

A

Hektoen enteric (HE) agar

21
Q

Isolation and growth of Corynebacterium
spp.

A

Loeffler medium

22
Q

For the selection and differentiation of
E. coli 0157:H7 in stool specimens

A

MacConkey sorbitol agar

23
Q

Selective differentiation of staphylococci

A

Mannitol salt agar

24
Q

Selective for Neisseria gonorrhoeae;
also supports the growth of Ureaplasma urealyticum and some
Mycoplasma spp.

A

NYC agar

25
Q

Selective isolation of aerobic gram-positive cocci and bacilli and anaerobic gram-positive cocci and gram-negative bacilli

A

Phenylethyl alcohol (PEA)
agar

26
Q

Enrichment and selective medium for isolation of Bordetella pertussis

A

Regan Lowe

27
Q

Selective for Salmonella and some
Shigella spp.

A

Salmonella-Shigella (SS)
agar

28
Q

Nonselective medium for the recovery of anaerobes and aerobes
Selective for Campylobacter spp. and
Helicobacter spp.

A

Schaedler agar

29
Q

Enrichment of isolation of Salmonella
spp.

A

Selenite broth

30
Q

Selective for Streptococcus pyogenes and Streptococcus agalactiae

A

Streptococcal selective agar (SSA)

31
Q

Selective for N. gonorrhoeae and
N. meningitidis.
Supports the growth of Francisella and
Brucella spp.

A

Thayer-Martin agar (TM)
(modified Thayer
Martin IMTM1)

32
Q

Supports growth of anaerobes, aerobes. microaerophilic, and fastidious microorganisms

A

Thioglycollate broth

33
Q

Selective and differential for Vibrio spp.

A

Thiosulfate citrate–bile salts (TCBS) agar

34
Q

Selective for Salmonella and Shigella spp., except Salmonella enterica
Typhi

A

Tetrathionate broth

35
Q

Selection and enrichment for Streptococcus agalactiae in female genital specimens

A

Todd-Hewitt broth
supplemented with antibiotics (LIM)

36
Q

Isolation and differentiation of Salmonella and Shigella spp. from other gram-negative enteric bacilli

A

Xylose lysine deoxycholate
(XLD) agar

37
Q

Enrichment broth used for subculturing various bacteria from primary agar plates

A

Trypticase soy broth
(TSB)

38
Q

Isolation and differentiation of lactose fermenting and non-lactose-fermenting enteric bacilli

A

MacConkey Agar

Plating Media for Routine Bacteriology (2 decks)

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