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Plating Media for Routine Bacteriology > Components/comments > Flashcards

Components/comments Flashcards

1
Q

Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria.

A

Bile esculin agar (BEA)

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2
Q

Contains azide to inhibit gram-negative bacteria, vancomycin to select for resistant gram-positive bacteria, and bile esculin to differentiate enterococci from other vancomycin-resistant bacteria that may grow

A

Bile esculin azide agar with vancomycin

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3
Q

Trypticase soy agar, Brucella agar, or beef heart infusion with 5% sheep blood

A

Blood Agar (BA)

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4
Q

Potato-glycerol-based medium enriched with 15%-20% defibrinated blood; contaminants inhibited by methicillin (final concentration of 2.5 um/mL)

A

Bordet-Gengou agar

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5
Q

Dextrose, pork brain, and heart dehydrated infusions

A

Brain-heart infusion agar
or broth

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6
Q

Yeast extract, agar, charcoal, and salts supplemented with L-cysteine HCI, ferric pyrophosphate, ACES buffer, and alpha-ketoglutarate

A

Buffered charcoal-yeast extract agar (BCYE)

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7
Q

BCYE supplemented with polymyxin B, vancomycin, and ansamycin to inhibit gram-negative bacteria, gram-positive bacteria, and yeast, respectively

A

Buffered charcoal-yeast extract (BCYE) agar with antibiotics

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8
Q

Bile salts, gentamicin, ticarcillin, polymyxin B, peptone, yeast extract

A

Burkholderia cepacia selective agar

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9
Q

Contains vancomycin (10 mg/L), trimethoprim
(5 mg/L), polymyxin B (2500 U/L), amphotericin B (2 mg/L), and cephalothin (15 mg/L) in a Brucella agar base with sheep blood

A

Campy- Blood Agar

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10
Q

Thioglycollate broth supplemented with increased agar concentration and antibiotics

A

Campylobacter thioglycollate broth

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11
Q

Tryptic soy broth with phenyl-ethyl alcohol, 5% sheep blood, and added nutrients

A

CDC* anaerobe 5% sheep blood agar

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12
Q

Blood-supplemented enrichment medium containing cefoperazone, vancomycin, and amphotericin to inhibit growth of most gram-negative bacteria, gram-positive bacteria, and yeast, respectively

A

Cefoperazone, vancomycin, amphotericin (CVA) medium

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13
Q

Peptone base with yeast extract, mannitol, and bile salts; supplemented with cefsulodin, irgasan, and novobiocin; neutral red and crystal violet indicators

A

Cefsulodin-irgasan-novobiocin (CIN) agar

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14
Q

Organism-specific nutrient base, selective supplements, and chromogenic substrate

A

Chromogenic media

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15
Q

Columbia agar base with 10 mg colistin per liter, 15 mg nalidixic acid per liter, and 5% sheep blood

A

Columbia colistin-nalidixic acid (CNA)
agar

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16
Q

Infusion agar base with 5% sheep blood; reduction of potassium tellurite by Corynebacterium diphtheriae produces black colonies

A

Cystine-tellurite blood agar

17
Q

Peptone base containing lactose; eosin Y and methylene blue as indicators

A

Eosin methylene blue (EMB) agar (Levine)

18
Q

Peptone-base broth with glucose and mannitol; sodium citrate and sodium deoxycholate act as inhibitory agents

A

Gram-negative broth (GN)

19
Q

Peptone-base agar with bile salts, lactose, sucrose, salicin, and ferric ammonium citrate; indicators include bromthymol blue and acid fuchsin

A

Hektoen enteric (HE) agar

20
Q

Animal tissue (heart muscle), dextrose, egg-and-beef serum, and sodium chloride

A

Loeffler medium

21
Q

Peptone base with lactose; gram-positive organisms inhibited by crystal violet and bile salts; neutral red as indicator

A

MacConkey agar

22
Q

A modification of MacConkey agar in which lactose has been replaced with D-sorbitol as the primary carbohydrate

A

MacConkey sorbitol agar

23
Q

Peptone base, mannitol, and phenol red indicator; salt concentration of 7.5% inhibits most bacteria

A

Mannitol salt agar

24
Q

Peptone agar base with cornstarch, supplemented with yeast dialysate, 3% hemoglobin, and horse plasma; antibiotic supplement includes vancomycin (2 ug/mL), colistin (5.5 ug/mL), amphotericin B (1.2 ug/mL), and trimethoprim (3 ug/mL)

A

New York City (NYC)
agar

25
Q

Nutrient agar base; phenylethyl alcohol inhibits growth of gram-negative organisms

A

Phenylethyl alcohol (PEA)
agar

26
Q

Charcoal agar supplemented with horse blood,
cephalexin, and amphotericin B

A

Regan Lowe

27
Q

Peptone base with lactose, ferric citrate, and sodium citrate; neutral red as indicator; inhibition of coliforms by brilliant green and bile salts

A

Salmonella-Shigella (SS) agar

28
Q

Peptone and soy protein base agar with yeast extract, dextrose, and buffers; addition of hemin, L-cysteine, and 5% blood enriches for anaerobes

A

Schaedler agar

29
Q

Peptone-base broth; sodium selenite toxic for most Enterobacteriaceae

A

Selenite broth

30
Q

Peptone and soy protein-base agar with lysed horse blood; vancomycin inhibits gram-positive organ-isms; polymyxin B and trimethoprim inhibit most gram-negative organisms

A

Skirrow agar

31
Q

Contains crystal violet, colistin, and trimethoprim-sulfamethoxazole in 5% sheep blood agar base

A

Streptococcal selective agar (SSA)

32
Q

Peptone-base broth; iodine and potassium iodide, bile salts, and sodium thiosulfate inhibit gram-positive organisms and Enterobacteriaceae

A

Tetrathionate broth

33
Q

Blood agar base enriched with hemoglobin and supplement B; contaminating organisms inhibited by colistin, nystatin, vancomycin, and trimethoprim

A

Thayer-Martin agar (TM)
(modified Thayer
Martin IMTM1)

34
Q

Pancreatic digest of casein, soy broth, and glucose enrich growth of most microorganisms; includes reducing agents thioglycollate, cystine, and sodium sulfite; semisolid medium with a low concentration of agar reducing oxygen diffusion in the medium

A

Thioglycollate broth

35
Q

Peptone base agar with yeast extract, bile salts, citrate, sucrose, ferric citrate, and sodium thiosulfate; bromthymol blue acts as indicator

A

Thiosulfate citrate–bile salts (TCBS) agar

36
Q

Supplemented with nalidixic acid and gentamicin or colistin for greater selectivity; thioglycollate and agar reduce redox potential

A

Todd-Hewitt broth supplemented with antibiotics (LIM)

37
Q

All-purpose enrichment broth that can support the growth of many fastidious and nonfastidious bacteria

A

Trypticase soy broth
(TSB)

38
Q

Yeast extract agar with lysine, xylose, lactose, sucrose, and ferric ammonium citrate; sodium deoxycholate inhibits gram-positive organisms; phenol red as indicator

A

Xylose lysine deoxycholate (XLD) agar

Plating Media for Routine Bacteriology (2 decks)

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