Components/comments

Question 1

Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria.

Answer 1

Bile esculin agar (BEA)

Question 2

Contains azide to inhibit gram-negative bacteria, vancomycin to select for resistant gram-positive bacteria, and bile esculin to differentiate enterococci from other vancomycin-resistant bacteria that may grow

Answer 2

Bile esculin azide agar with vancomycin

Question 3

Trypticase soy agar, Brucella agar, or beef heart infusion with 5% sheep blood

Answer 3

Blood Agar (BA)

Question 4

Potato-glycerol-based medium enriched with 15%-20% defibrinated blood; contaminants inhibited by methicillin (final concentration of 2.5 um/mL)

Answer 4

Bordet-Gengou agar

Question 5

Dextrose, pork brain, and heart dehydrated infusions

Answer 5

Brain-heart infusion agar
or broth

Question 6

Yeast extract, agar, charcoal, and salts supplemented with L-cysteine HCI, ferric pyrophosphate, ACES buffer, and alpha-ketoglutarate

Answer 6

Buffered charcoal-yeast extract agar (BCYE)

Question 7

BCYE supplemented with polymyxin B, vancomycin, and ansamycin to inhibit gram-negative bacteria, gram-positive bacteria, and yeast, respectively

Answer 7

Buffered charcoal-yeast extract (BCYE) agar with antibiotics

Question 8

Bile salts, gentamicin, ticarcillin, polymyxin B, peptone, yeast extract

Answer 8

Burkholderia cepacia selective agar

Question 9

Contains vancomycin (10 mg/L), trimethoprim
(5 mg/L), polymyxin B (2500 U/L), amphotericin B (2 mg/L), and cephalothin (15 mg/L) in a Brucella agar base with sheep blood

Answer 9

Campy- Blood Agar

Question 10

Thioglycollate broth supplemented with increased agar concentration and antibiotics

Answer 10

Campylobacter thioglycollate broth

Question 11

Tryptic soy broth with phenyl-ethyl alcohol, 5% sheep blood, and added nutrients

Answer 11

CDC* anaerobe 5% sheep blood agar

Question 12

Blood-supplemented enrichment medium containing cefoperazone, vancomycin, and amphotericin to inhibit growth of most gram-negative bacteria, gram-positive bacteria, and yeast, respectively

Answer 12

Cefoperazone, vancomycin, amphotericin (CVA) medium

Question 13

Peptone base with yeast extract, mannitol, and bile salts; supplemented with cefsulodin, irgasan, and novobiocin; neutral red and crystal violet indicators

Answer 13

Cefsulodin-irgasan-novobiocin (CIN) agar

Question 14

Organism-specific nutrient base, selective supplements, and chromogenic substrate

Answer 14

Chromogenic media

Question 15

Columbia agar base with 10 mg colistin per liter, 15 mg nalidixic acid per liter, and 5% sheep blood

Answer 15

Columbia colistin-nalidixic acid (CNA)
agar

Question 16

Infusion agar base with 5% sheep blood; reduction of potassium tellurite by Corynebacterium diphtheriae produces black colonies

Answer 16

Cystine-tellurite blood agar

Question 17

Peptone base containing lactose; eosin Y and methylene blue as indicators

Answer 17

Eosin methylene blue (EMB) agar (Levine)

Question 18

Peptone-base broth with glucose and mannitol; sodium citrate and sodium deoxycholate act as inhibitory agents

Answer 18

Gram-negative broth (GN)

Question 19

Peptone-base agar with bile salts, lactose, sucrose, salicin, and ferric ammonium citrate; indicators include bromthymol blue and acid fuchsin

Answer 19

Hektoen enteric (HE) agar

Question 20

Animal tissue (heart muscle), dextrose, egg-and-beef serum, and sodium chloride

Answer 20

Loeffler medium

Question 21

Peptone base with lactose; gram-positive organisms inhibited by crystal violet and bile salts; neutral red as indicator

Answer 21

MacConkey agar

Question 22

A modification of MacConkey agar in which lactose has been replaced with D-sorbitol as the primary carbohydrate

Answer 22

MacConkey sorbitol agar

Question 23

Peptone base, mannitol, and phenol red indicator; salt concentration of 7.5% inhibits most bacteria

Answer 23

Mannitol salt agar

Question 24

Peptone agar base with cornstarch, supplemented with yeast dialysate, 3% hemoglobin, and horse plasma; antibiotic supplement includes vancomycin (2 ug/mL), colistin (5.5 ug/mL), amphotericin B (1.2 ug/mL), and trimethoprim (3 ug/mL)

Answer 24

New York City (NYC)
agar

Question 25

Nutrient agar base; phenylethyl alcohol inhibits growth of gram-negative organisms

Answer 25

Phenylethyl alcohol (PEA) agar

Question 26

Charcoal agar supplemented with horse blood, cephalexin, and amphotericin B

Answer 26

Regan Lowe

Question 27

Peptone base with lactose, ferric citrate, and sodium citrate; neutral red as indicator; inhibition of coliforms by brilliant green and bile salts

Answer 27

Salmonella-Shigella (SS) agar

Question 28

Peptone and soy protein base agar with yeast extract, dextrose, and buffers; addition of hemin, L-cysteine, and 5% blood enriches for anaerobes

Answer 28

Schaedler agar

Question 29

Peptone-base broth; sodium selenite toxic for most Enterobacteriaceae

Answer 29

Selenite broth

Question 30

Peptone and soy protein-base agar with lysed horse blood; vancomycin inhibits gram-positive organ-isms; polymyxin B and trimethoprim inhibit most gram-negative organisms

Answer 30

Skirrow agar

Question 31

Contains crystal violet, colistin, and trimethoprim-sulfamethoxazole in 5% sheep blood agar base

Answer 31

Streptococcal selective agar (SSA)

Question 32

Peptone-base broth; iodine and potassium iodide, bile salts, and sodium thiosulfate inhibit gram-positive organisms and Enterobacteriaceae

Answer 32

Tetrathionate broth

Question 33

Blood agar base enriched with hemoglobin and supplement B; contaminating organisms inhibited by colistin, nystatin, vancomycin, and trimethoprim

Answer 33

Thayer-Martin agar (TM) (modified Thayer Martin IMTM1)

Question 34

Pancreatic digest of casein, soy broth, and glucose enrich growth of most microorganisms; includes reducing agents thioglycollate, cystine, and sodium sulfite; semisolid medium with a low concentration of agar reducing oxygen diffusion in the medium

Answer 34

Thioglycollate broth

Question 35

Peptone base agar with yeast extract, bile salts, citrate, sucrose, ferric citrate, and sodium thiosulfate; bromthymol blue acts as indicator

Answer 35

Thiosulfate citrate--bile salts (TCBS) agar

Question 36

Supplemented with nalidixic acid and gentamicin or colistin for greater selectivity; thioglycollate and agar reduce redox potential

Answer 36

Todd-Hewitt broth supplemented with antibiotics (LIM)

Question 37

All-purpose enrichment broth that can support the growth of many fastidious and nonfastidious bacteria

Answer 37

Trypticase soy broth (TSB)

Question 38

Yeast extract agar with lysine, xylose, lactose, sucrose, and ferric ammonium citrate; sodium deoxycholate inhibits gram-positive organisms; phenol red as indicator

Answer 38

Xylose lysine deoxycholate (XLD) agar