Primary Culture Techniques Flashcards
1
Q
Describe the characteristics of primary cells.
A
- Cells derived directly from tissues
- Interpatient variability
- Finite lifespan
- Cells divide and/or differentiate
- Cells carry out normal functions
2
Q
What are the 2 broad classes of primary cells?
A
Haemopoietic and non-haemopoietic
3
Q
Describe how cells are disaggregated.
A
- Cells allowed to migrate out of an explant
- Mechanical dissociation
- Enzymatic dissociation
- Haemopoietic cells do not need to be disaggregated
4
Q
Describe 3 sources of stem cells.
A
- Bone marrow aspirate
- Umbilical cord blood
- Mobilised peripheral blood
