Microscopy Flashcards
1
Q
Which are the two parameters that must be controlled in live imaging?
A
Temperature and CO2 concentration
2
Q
Magnification and numerical apperture (NA) are synonyms. True or false?
A
False
Magnification is the capability of a specific objective to increase the size of the specimen when observed through the detector
The NA aperture determines resolution, not magnification
3
Q
Which microscopy technique is related to the “Stokes Shift”?
A
Fluorescence microscopy
4
Q
What is “Stokes shift”?
A
The difference between the wavelength of excitation and emission
5
Q
What is the main advantage and disadvantage of confocal microscopy?
A
Advantage - higher Z-resolution and reduced out of focus blur
Disadvantage - only a small volume can be visualised by confocal microscopes at once
