Presentation 1

Question 1

Complement

Answer 1

cascade consists of interactions between several complement proteins resulting in activation of 3 pathways

Question 2

zymogen cascade

Answer 2

detection of proteins activates zymogen
triggers cascade of proteolysis
zymogens are sequentially activated to = active proteases, cleave and continue cascade

Question 3

zymogen

Answer 3

an inactive protein precursor of an enzyme secreted by living cells and converted (as by a kinase or an acid) into an active form. called also proenzyme.

Question 4

cascade result

Answer 4

amplifies signal resulting in activation of phagocytosis, MAC, inflammatory response

Question 5

three pathways

Answer 5

The classical pathway
The alternative pathway
the lectin pathway

all 3 result in production of C3 convertase that cleaves C3 to release C3a and C3b which

Question 6

classical complement

Answer 6

activated via anitbody binding

Question 7

CC effector function

Answer 7

opsonization
leukocyte/microglial recruitment
target
membraneoltyic pore in pathogen cell membrane = death

Question 8

mechanism of action

Answer 8

clearing of target antigen
CDC pathway - complement dependent cytotoxicity
complement receptor CR dependent phago. cyto

Question 9

C1q complex

Answer 9

six globular heads helped together by collagen like tails
C1r and C1s composed

Question 10

initiation of CC

Answer 10

binding C1q surface = conformational change to R and S complexes = activation of autocatalytic activity in R, R then cleaves S = active serine protease

Question 11

formation C3 convertase

Answer 11

activated S binds C4 and cleaves = C4a and C4b
4b binds covalently to surface of protein and bindi C2, cleaved by C1 into C2a and b
C2a and C4b remain on surface acting as C3 convertase, C3 convertase cleaves C3 into C3a and C3b
C3a=local inflammatory response

Question 12

C5 convertase and MAC

Answer 12

C3b binds C4bC2B complex = C5 convertase