preparing blood samples Flashcards
1
Q
how to make defibrinated blood
A
Shake flask with blood and small glass beads to change fibrinogen into fibrin.
2
Q
examples of anticoagulants and how they work
A
sodium citrate
EDTA(ETHYLENE DIAMINE TETRAACETIC ACID), they both bind calcium ions
3
Q
effects of heparin
A
prevents blood from coagulating by catalysing the thrombin-antithrombin reaction
4
Q
how to obtain blood serum
A
centrifuge defibrinated blood (after it has been shaken with glass beads)
5
Q
why is the process of defibrination is of clinical importance?
A
the blood getting into the body cavities is defibrinated due to the constant moving of the organs so it does not coagulate
6
Q
at what speed the blood has to be centrifuged to leave sediment and supernatant of clear blood plasma?
A
3000 rpm for 10 min.
