determining blood clotting time

Question 1

Lee White method?

Answer 1

pour 1-2 ml blood in tube

tilt the tubes at intervals of three minutes to see if the blood coagulates

Question 2

what is blood clotting time?

Answer 2

the time elapsed from the beginning of blood sampling to the appearance of the first fibrin thread.

Question 3

what is hemostasis?

Answer 3

to prevent/ stop the bleeding

Question 4

what are the three steps of hemostasis?

Answer 4

1. vascular reaction
2. thrombocyte reaction
3. thrombolysis

Question 5

what can the blood clotting time inform us about?

Answer 5

the presence and activity of thrombocytes (platelets) and clotting factors (fibrinogen)

Question 6

in which 2 ways the blood clotting time will be examined in the course?

Answer 6

on the basis of the appearance of the first fibrin thread and using the Lee White method

Question 7

how can you examine the blood clotting time by the appearance of the first fibrin thread?

Answer 7

drip some blood onto a watch glass.
at intervals of 30 seconds draw a thin glass rod through the blood to see if there is an appearance of the first fibrin thread

Question 8

why do we need to check the blood clotting time also at water bath in 37-degree celsius?

Answer 8

since the velocity of enzymatic reactions is highest at body temp. blood clotting time is longer at room temp.

Question 9

which test tube should be used for the lee white method

Answer 9

Wasserman tube