Preparing a Streak Plate From a Mixed Broth Culture

Question 1

What is the 1st step in creating a streak plate from a mixed broth culture?

Answer 1

Label the bottom of the plate with the name of the bacterial mixture, your initials, the date, and section

Question 2

What is the 2nd step in creating a streak plate from a mixed broth culture?

Answer 2

Sanitize working area with microbicide

Question 3

What is the 3rd step in creating a streak plate from a mixed broth culture?

Answer 3

Turn on the Bunsen burner

Question 4

What is the 4th step in creating a streak plate from a mixed broth culture?

Answer 4

Suspend the cells in the broth tube by gently shaking the tube

Question 5

What is the 5th step in creating a streak plate from a mixed broth culture?

Answer 5

Sterilize the inoculating loop through the flame until the loop becomes red. Allow loop to cool

Question 6

What is the 6th step in creating a streak plate from a mixed broth culture?

Answer 6

With the inoculating loop in my right hand, pick up the broth culture tube in my left. Using my right pinky, remove the cap from the broth culture tube

Question 7

What is the 7th step in creating a streak plate from a mixed broth culture?

Answer 7

Quickly pass the tube at an angle through the flame 3 times.

Question 8

What is the 8th step in creating a streak plate from a mixed broth culture?

Answer 8

Insert the cooled loop into the broth culture to obtain a loop-ful of broth culture

Question 9

What is the 9th step in creating a streak plate from a mixed broth culture?

Answer 9

Holding the inoculating loop away from the flame, quickly pass the mouth of the tube at an angle through the flame 3 times. Replace the cap and set aside.

Question 10

What is the 11th step in creating a streak plate from a mixed broth culture?

Answer 10

With the agar bottom down, lift one side of the Petri plate lid just enough so you can streak out the plate

Question 11

What is the 12th step in creating a streak plate from a mixed broth culture?

Answer 11

Spread the broth over 1/3 of the plate in a back and forth motion

Question 12

What is the 13th step in creating a streak plate from a mixed broth culture?

Answer 12

Sterilize the loop in the flame until it becomes red. Allow loop to cool

Question 13

What is the 14th step in creating a streak plate from a mixed broth culture?

Answer 13

Move the loop time through the last few streaks in the first quadrant, and without lifting the loop streak another 1/3 of the plate

Question 14

What is the 15th step in creating a streak plate from a mixed broth culture?

Answer 14

Repeat last two steps until the plate is covered

Question 15

What is the 16th step in creating a streak plate from a mixed broth culture?

Answer 15

Sterilize the loop in the flame until it becomes red. Set aside loop and incubate plate

Question 16

What is the 17th step in creating a streak plate from a mixed broth culture?

Answer 16

Disinfect the work space with microbicide