preparing a specimen

Question 1

outline process of preparing a specimen

Answer 1

clean slide, drop of liquid on slide, obtain specimen, place specimen, add cover slip gently with needle

Question 2

wet mount for examining plant cell

Answer 2

tap water

Question 3

wet mount for examining animal cell

Answer 3

physiological saline

Question 4

what happens if plain water used as wet mount when examining animal cells

Answer 4

cells will explode from osmotic pressure as no cell wall for structural support

Question 5

how would you obtain the skin of an onion bulb

Answer 5

bend single layer of onion towards shiny side, after it snaps pull gently and transparent layer of skin will appear

Question 6

important things to get right

Answer 6

thickness of specimen, no air bubbles, use of stain to clarify view, visibility and magnification

Question 7

how to obtain cheek cells- epithelial

Answer 7

gently rub toothpick on inside of mouth, swirl in p.saline on slide