pag 1 microscopy

Question 1

magnification of objective lenses used

Answer 1

10x and 40x

Question 2

stain used

Answer 2

Leishman’s stain

Question 3

why must you focus stage down

Answer 3

to avoid broken cover slip

Question 4

how to avoid contamination of slide surface

Answer 4

handle slides by edges

Question 5

reason for gently shaking sample of blood

Answer 5

to re-suspend the cells

Question 6

purpose of hypochlorite

Answer 6

clean contaminated equipment

Question 7

how to smear the blood

Answer 7

2nd slide @ 45’, push backwards until touches drop of blood, then pull towards far end of side

Question 8

why is it important that the second slide makes the briefest possible contact with the drop of blood

Answer 8

to prevent to much from being collected and far too many cells being spread on the slide

Question 9

why is the smear left to air dry

Answer 9

so the cells will stick to the slide

Question 10

why is ethanol poured onto dry smear (for 2min)

Answer 10

to fix and dehydrate the cells

Question 11

where is the cover slip placed

Answer 11

at end where smear is thinnest

Question 12

purpose to using x10 objective

Answer 12

to focus the microscope and identify right part of smear