PREPARATION OF RED CELL SUSPENSION

Question 1

red cells remaining after washing with a volume of comparable solution using a method known to remove almost all of the plasma

Answer 1

Washed Red Blood Cells

Question 2

Liquid portion of the
whole blood containing water,
electrolytes, glucose, fats,
proteins, and gases.

Answer 2

Plasma

Question 3

cells freed of plasma or serum
by repeated centrifugation
through fresh volume of
normal saline.

Answer 3

Washed Cells

Question 4

preferred anticoagulant
during preparation of red cell
suspension

Answer 4

EDTA

Question 5

What should a labelled test tubes contain?

Answer 5

I. Plasma/Patient’s identification
II. Washed cells/Patient’s identification
III. 5% Red blood cell suspension/patient’s identification

Question 6

In preparing an exact 5% washed red cell suspension, it will be centrifuged at?

Answer 6

for 1 minute at 3400 rpm

Question 7

How many times do we need to repeat the washes?

Answer 7

three or four washes.

Question 8

What is the formula for red cell suspension?

Answer 8

amount of washed packed RBCs/total volume (100)

Question 9

Total volume is equal to?

Answer 9

amount of washed packed RBCs + amount of NSS to be added

Question 10

2% of RCS

Answer 10

4. 90 ml NSS
   0.10 ml Washed cells

Question 11

3% RCS

Answer 11

4.85 ml NSS
0.15 ml Washed cells

Question 12

4% RCS

Answer 12

4.80 ml NSS
0.20 ml Washed cells

Question 13

5% RCS

Answer 13

4.75 ml NSS
0.25 ml Washed cells