- SODIUM POLYANETHOL SULFONATE (SPS) - ACID CITRATE DEXTROSE

PRELIM LAB: BLOOD COLLECTION TUBES & TESTS Flashcards by Chin Lys

Used with both ETS and syringe method of obtaining blood specimens

EVACUATED TUBES

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Contains pre-measured vacuum

EVACUATED TUBES

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color-coded to identify a type of additive, absence of additive, or special tube property

Tube stoppers

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Made of glass or plastic

EVACUATED TUBES

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Used to collect tiny amounts of blood obtained from capillary punctures

MICROCOLLECTION TUBES

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Most have color-coded bodies or stoppers that correspond to color-coding of ETS blood collection tubes

MICROCOLLECTION TUBES

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substances you add in the evacuated tubes

ADDITIVE

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type of additive you add in tubes to prevent clotting

ANTICOAGULANT

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consumes

CHELATES

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attaches to

BINDS

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ADDITIVE

YELLOW

SODIUM POLYANETHOL SULFONATE (SPS)
ACID CITRATE DEXTROSE

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MODE OF ACTION

YELLOW

Prevent coagulation by binding
calcium

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LABORATORY USAGE

YELLOW

MICROBIOLOGY (Blood Cultures)
BLOOD BANKING (Blood bank studies, HLA phenotyping, and DNA & paternity testing)

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INVERSIONS

YELLOW

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LABORATORY USE

LIGHT BLUE

HEMATOLOGY (Coagulation Studies)
- PT (prothrombin time) and aPTT (activated partial thromboplastin time): SENSITIVE TESTS

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ADDITIVE

LIGHT BLUE

3.2% BUFFERED SODIUM CITRATE
- Blood to anticoagulant
ratio (9:1)

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MODE OF ACTION

LIGHT BLUE

Prevent coagulation by chelating
calcium

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INVERSIONS

LIGHT BLUE

3-4 GENTLE inversions

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ADDITIVE

RED

SILICONE-COATED (Glass)

CLOT ACTIVATION (SILICONE-COATED) – Plastic

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MODE OF ACTION

RED

Promotes blood clotting

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LABORATORY USE

RED

CHEMISTRY (serum chemistry determination)
BLOOD BANK (routine blood donor screening)
IMMUNOSEROLOGY (diagnostic testing for infectious disease)

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INVERSION

RED

5 inversions if with clot activator
No inversion for glass tubes

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ADDITIVE

GOLD PLASTIC

NO ADDITIVE GEL SEPARATOR
(Thixotropic gel) TG has a heavier specific
gravity than serum but is lighter than RBC

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MODE OF ACTION

GOLD PLASTIC

Promotes blood clotting
- Blood clotting time: 30 minutes

# LABORATORY USE GOLD PLASTIC

CHEMISTRY (serum chemistry determination) BLOOD BANK (routine blood donor screening) IMMUNOSEROLOGY (diagnostic testing for infectious disease)

# INVERSION GOLD PLASTIC

5 inversions

# ADDITIVE LIGHT GREEN

LITHIUM HEPARIN GEL SEPARATOR

# MODE OF ACTION LIGHT GREEN

Inhibits thrombin formation to prevent clotting

# LABORATORY USE LIGHT GREEN

CHEMISTRY (plasma determination in chemistry)

# INVERSION LIGHT GREEN

8-10 inversions

# ADDITIVE GREEN

15-20 u/mL LITHIUM HEPARIN or SODIUM HEPARIN

# MODE OF ACTION GREEN

Inhibits thrombin formation to prevent clotting

# LABORATORY USE GREEN

CHEMISTRY (blood gas analysis) HEMATOLOGY (osmotic fragility test)

# INVERSION GREEN

8-10 inversions

# ADDITIVE LAVENDER

1.5 mg/mL of blood of ETHYLENEDIAMINE TETRAACETIC ACID (EDTA) - Must be tested within a 4-hour duration of being used for optimal results

# MODE OF ACTION LAVENDER

Inhibits blood coagulation by chelating calcium ions

# LABORATORY USE LAVENDER

CHEMISTRY (HBA1C) - HBA1C (glycosylated hemoglobin): most sensitive test for diabetic patients as it could trace back one’s average blood sugar up to 3 months prior to test HEMATOLOGY (routine CBC, ESR) BLOOD BANKING (routine immunohematology testing and donor screening)

# INVERSION LAVENDER

8-10 inversions

most sensitive test for diabetic patients as it could trace back one’s average blood sugar up to 3 months prior to test

HBA1C (glycosylated hemoglobin)

# ADDITIVE GRAY Anticoagulant:

POTASSIUM OXALATE

# ADDITIVE GRAY Antiglycolytic agent (stops cells from producing sodium-glucose):

SODIUM FLUORIDE

GRAY Inhibits bacterial growth. Can be stored for 3 DAYS

SODIUM FLUORIDE

GRAY decreases glucose concentration by up to 10 mg/dL/hour

Glycolysis

# MODE OF ACTION GRAY

Oxalate prevents clotting by binding calcium

# MODE OF ACTION GRAY preserves glucose for up to and inhibits bacterial growth

SODIUM FLUORIDE

# LABORATORY USE GRAY

CHEMISTRY (glucose determination) - IDEAL FOR FBS

# INVERSION GRAY

8-10 inversions

# ADDITIVE ORANGE

THROMBIN

# MODE OF ACTION ORANGE

Clot activator - Blood clotting time: 5 mins

# LABORATORY USE ORANGE

CHEMISTRY (STAT determination)

# INVERSION ORANGE

8 inversion

# ADDITIVE ROYAL BLUE

NONE (red label) K2EDT (lavender label) HEPARIN (green label)

# ADDITIVE TAN

SODIUM HEPARIN (Glass) K2EDTA (Plastic)

# ADDITIVE PINK

Spray-coated K2EDTA (Plastic)

# MODE OF ACTION ROYAL BLUE TAN PINK

EDTA: prevents clotting by binding calcium forming insoluble salt HEPARIN: prevents clotting by inhibiting thrombin

# INVERSION ROYAL BLUE TAN PINK

# LABORATOTY USE ROYAL BLUE

CHEMISTRY (trace elements, toxicology)

# LABORATORY USE TAN

CHEMISTRY (lead determination)

# LABORATORY USE PINK

BLOOD BANK (routine immunohematology testing and blood donor screening) SPECIAL TUBE in BB