PRELIM - ENZYMES-L1 Flashcards
INHIBITORS:
- Physically bind to the ACTIVE Site of an enzyme and COMPETE WITH THE SUBSTRATE FOR THE ACTIVE SITE
COMPETITIVE INHIBITION
INHIBITORS:
- Binds an enzyme at a place OTHER THAN THE ACTIVE SITE AND MAY BE REVERSIBLE IN THAT SOME NATURALLY PRESENT METABOLIC SUBSTANCES COMBINE REVERSIBLY WITH CERTAIN ENZYMES.
NON COMPETITIVE INHIBITION
TRUE OR FALSE
- NON COMPETITIVE INHIBITORS may also be IRREVERSIBLE if the INHIBITOR destroys part of the enzyme involved in catalytic activity.
TRUE
INHIBITOR:
- This inhibitor binds to the ES complex—increasing substrate concentration results in more ES complexes to which the inhibitor binds and, thereby, increases the inhibition.
UNCOMPETITIVE INHIBITION
CLINICAL SIGNIFICANCE:
PROSTATIC CARCINOMA
ACID PHOSPHATASE
CLINICAL SIGNIFICANCE:
PROSTATIC CARCINOMA
ACID PHOSPHATSE
CLINICAL SIGNIFICANCE:
HEPATIC DISORDER
ALANINE AMINOTRANSFERASE
CLINICAL SIGNIFICANCE of ALANINE AMINOTRANSFERASE
HEPATIC DISORDER
What is the CLINICAL SIGNIFICANCE OF ALKALINE PHOSPHATASE?
HEPATIC DISORDER
BONE DISORDER
CLINICAL SIGNIFICANCE:
HEPATIC DISORDER & BONE DISORDER
What enzyme?
ALKALINE PHOSPHATASE
CLINICAL SIGNIFICANCE OF AMYLASE?
ACUTE PANCREATITIS
CLINICAL SIGNIFICANCE: ACUTE PANCREATITIS
AMYLASE
CLINICAL SIGNIFICANCE OF Angiotensin-converting enzyme (ACE)
BLOOD PRESSURE REGULATION
CLINICAL SIGNIFICANCE: Blood pressure regulation
ACE
What is the CLINICAL SIGNIFICANCE OF AST?
MYOCARDIAL INFARCTION
HEPATIC DISORDER
SKELETAL MUSCLE DISORDER
CLINICAL SIGNIFICANCE:
MYOCARDIAL INFARCTION
HEPATIC DISORDER
SKELETAL MUSCLE DISORDER
ASPARTATE AMINOTRANSFERASE
CLINICAL SIGNIFICANCE of CREATINE KINASE?
MYOCARDIAL INFARCTION
SKELETAL MUSCLE DISORDER
CLINICAL SIGNIFICANCE:
MYOCARDIAL INFARCTION
SKELETAL MUSCLE DISORDER
- What enzyme?
CREATINE KINASE
What is the CLINICAL SIGNIFICANCE of G-6-PD?
DRUG-INDUCED HEMOLYTIC ANEMIA
CLINICAL SIGNIFICANCE: DRUG-INDUCED HEMOLYTIC ANEMIA
- What enzyme?
GLUCOSE-6-PHOSPHATE DEHYDROGENASE
What is the CLINICAL SIGNIFICANCE OF GGT?
HEPATIC DISORDER
CLINICAL SIGNIFICANCE: HEPATIC DISORDER
- What enzyme?
GAMMA-GLUTAMYLTRANSFERASE
What is the CLINICAL SIGNIFICANCE OF LACTATE DEHYDROGENASE?
MYOCARDIAL INFARCTION
HEPATIC DISORDER
HEMOLYSIS
CARCINOMA
CLINICAL SIGNIFICANCE: MYOCARDIAL INFARCTION
HEPATIC DISORDER
HEMOLYSIS
CARCINOMA
- What enzyme?
LACTATE DEHYDROGENASE
What is the CLINICAL SIGNIFICANCE OF LIPASE?
ACUTE PANCREATITIS
What is the CLINICAL SIGNIFICANCE OF 5’-NUCLEOTIDASE?
HEPATIC DISORDER
CLINICAL SIGNIFICANCE: HEPATIC DISORDER
- What enzyme?
GGT
What is the CLINICAL SIGNIFICANCE of PSEUDOCHOLINESTERASE?
ORGANOPHOSPHATE POISONING
GENETIC VARIANTS
HEPATIC DISORDER
SUXAMETHONIUM SENSITIVITY
CLINICAL SIGNIFICANCE:
ORGANOPHOSPHATE POISONING
GENETIC VARIANTS
HEPATIC DISORDER
SUXAMETHONIUM SENSITIVITY
- What enzyme?
PSEUDOCHOLINESTERASE (PChE)
ISOENZYME: CK MM
what are the CONDITIONS ASSOCIATED?
MYOCARDIAL INFARCTION
SKELETAL MUSCLE DISORDER
POLYMYOSITIS
HYPOTHYROIDISM
MALIGNANT HYPERTHERMIA
PHYSICAL ACTIVITY
INTRAMUSCULAR INJECTION
ISOENZYME: CK-MB
what are the CONDITIONS ASSOCIATED?
MYOCARDIAL INFARCTION
MYOCARDIAL INJURY
ISCHEMIA
ANGINA
INFLAMMATORY HEART DISEASE
CARDIAC SURGERY
DUCHENNE-TYPE MUSCULAR DYSTROPHY
POLYMYOSITIS
MALIGNANT HYPERTHERMIA
REYE’S SYNDROME
ROCKY MOUNTAIN SPOTTED
CARBON MONOXIDE POISONING
ISOENZYME: CK-BB
what are the CONDITIONS ASSOCIATED?
CENTRAL NERVOUS SYSTEM SHOCK
ANOXIC ENCEPHALOPATHY
CEREBROVASCULAR ACCIDENT
SEIZURE
PLACENTAL OR UTERINE TRAUMA
CARCINOMA
REYE’S SYNDROME
CARBON MONOXIDE POISONING
MALIGNANT HYPERTHERMIA
ACUTE AND CHRONIC RENAL FAILURE
TISSUE SOURCES OF CK-BB?
BRAIN
BLADDER
LUNG
PROSTATE
UTERUS
COLON
STOMACH
THYROID
What are the TISSUE SOURCES OF CK-MB?
HEART
SKELETAL MUSCLE
WHAT ARE THE TISSUE SOURCES OF CK-MB
HEART & SKELETAL MUSCLE
TISSUE SOURCES OF CK-MM?
HEART & SKELETAL MUSCLE
Major isoenzyme fraction found in striated muscle and normal serum.
CK-MM
3 ISOENZYME: CK-BB
BRAIN TYPE
3 ISOENZYME: CK-MB
HYBRID TYPE
3 ISOENZYME: CK-MM
MUSCLE TYPE
ISOENZYME that migrate fastest toward the ANODE
CK-BB (CK1)
The major isoenzyme in the sera of HEALTHY PEOPLE is?
CK-MM
ISOENZYME OF CK from CATHODAL-ANODAL
MI>MM>MACRO>MB>BB
CK-BB short half-life:
1-5 hours
REFERENCE RANGE OF CK:
MALES:
FEMALES:
(This is from BISHOP)
Males: 46 to 171 U/L (37°C) (0.8 to 2.9 µkat/L)
Females: 34 to 145 U/L (37°C) (0.6 to 2.4 µkat/L)
CK-MB: <5% total CK
