LABORATORY_ELECTROLYTES Flashcards
MOST ABUNDANT CATION IN EXTRACELLULAR FLUID
SODIUM
SPECIMEN FOR SODIUM:
SERUM
PLASMA (lithium, ammonium heparin & lithium oxalate)
SWEAT
24 HR URINE
METHODS : SODIUM
FLAME EMISSION SPECTROPHOTOMETRY
ION SELECTIVE ELECTRODE
ATOMIC ABSORPTION SPECTROPHOTOMETRY
2 TYPES OF ISE in SODIUM TEST:
DIRECT - provides undiluted sample
INDIRECT - used diluted sample
Sources of ERROR in ION SELECTIVE ELECTRODE METHOD for SODIUM TEST?
PROTEIN BUILDUP
MOST ROUTINELY USED method in SODIUM TESTING:
ION SELECTIVE METHOD
Sample volume for SODIUM?
10 uL
INCUBATION TIME FOR SODIUM?
5 MINUTES
TEMP FOR SODIUM TEST?
30 C
REFERENCE RANGE OF SODIUM:
SERUM: 135-145 mmol/L
URINE (24hr) : 40-220 mmol/d
CEREBROSPINAL FLUID : 135-150 mmol/L
It is the MAJOR INTRACELLULAR CATION in the body
POTASSIUM