Preclinical Research

Question 1

Preclinical research

Answer 1

Research conducted before direct experimental treatment or observation of human subjects

Question 2

Main objectives of preclinical research

Answer 2

Structure and function relationships

Question 3

What is LD50

Answer 3

Lethal dose

Question 4

How can RNA expression be studied

Answer 4

PCR
Microarrays

Question 5

How can protein structure and quantification be studied

Answer 5

ELISA
mass spectrometry

Question 6

How can molecular identity and structure be determined

Answer 6

X ray crystallography
Nuclear magnetic resonance
Cryo electron microscopy

Question 7

How can protein function and expression be assessed

Answer 7

Activity
Substrate and product levels
Enzyme products
Formation and depletion rates

Question 8

How can cell or tissue structure be studied

Answer 8

Histology
Immuno fluorescence
Flow cytometry
Electrophysiology
Motility
Contractility
Permeability
Whole organ perfusion

Question 9

Organoid

Answer 9

3-dimensional mass of tissue that is made by growing stem cells (cells from which other types of cells develop)

Question 10

Metabolomics

Answer 10

measurement of all metabolites and low-molecular-weight molecules in a biological specimen