Pre-Midterm Flashcards
What is phyisology
branch of biology relating to the function of organs and organ systems, and how they work within the biological body to respond to challenges
Plant Physiology
The study of how different parts of plants function for many aspects of plant life.
Translocation in the Phloem
Source to sink
Chloroplast
powerhouse for plant life
C4 Photosynthesis
A CO2 pump, fast photosynthesis of C4 plants under high light intensity and high temperature
Phytohormones
regulation of many aspects in all stages of the plant life cycle
Water balance
important properties of water molecules
How to pull water through the xylem
Water potential
Movement of water in plants
water moves from soil to root
water gets through the root
water moves up through the xylem
water moves from leaf to air
History- the study of plant water relations
Malpighi and Grew (1670-1680s)
Observations of plant conducting tissues
They found out plant structures which are similar to animal vasculature
Important properties of water
water is cohesive
water is an excellent solvent
water can dissociate into ions
water has a high latent heat of vaporization
Water has a high tensile strength
Water is cohesive
No net charge to a water molecule, however electronegative
Electronegative
Attract the electrons of the covalent bond
Hydrogen bonding and polar structures
Good solvents for ionic substances, sugar and proteins
Water has a high latent heat of vaporization
The heat required to change one mole of liquid at its boiling point under standard atmospheric pressure
44 kJ needed to cause 1 mole water to go from liquid to vapor state
Tensile strength
Ability to resist a pulling force of molecules before breaking their bonds
Cohesion
a molecule is attracted to the same molecule
Adhesion
a molecule is attracted to the other type of substances
Surface tension
the property of liquid surfaces which allows it to resist an external force, due to the cohesive nature of its molecules
How do plants bring water from the roots to the shoots?
Capillary action (Capillarity)
Water potential in Plants
Ability of water molecule to move freely in solution
Measurement of the potential energy in water
Water movement
high to low water potential
Chemical potential
Quantitative expression of the free energy associated with a substance
Water potential consists of 3 components
Solute potential
Pressure potential
Gravity potential
The major factors influencing the water potential in plants
Concentration
Pressure
Gravity
-sign for solute potential
Reduction of the water potential dissolved solutes
Pressure potential
effect of hydrostatic potential
Turgor pressure
+ hydrostatic pressure within cells (pressure potential >0 MPa)
Supports plants cells and tissues
water limitation lowers turgor pressure
Tension
- hydrostatic pressure, frequently develop in xylem
Pressure potential <0 MPa
Gravity potential in plants
plant height is generally to short for gravity potential to make a difference with water potential
Unless it is something tall like trees
Why is water potential important?
Cell growth, photosynthesis and crop productivity are highly influenced by water potential
Scholander pressure chamber
The pressure chamber method for measuring plant water potential
How to pull water through the xylem:
Cohesion, adhesion and surface tension
Water potential
Ability of water molecule to move freely in solution
Important mechanisms for water movement in plants
osmosis “water follows sugar”
Turgor pressure and plasmolysis
Solute potential
The solute potential is reduced when solutes are added to an aqeuous system
Water moves from 0 to negative number
Osmosis
phenomenon of water flow across a semi-permeable membrane
Sucrose cannot pass through the membrane, water moves across membranes
Important for water movement in plants, water borrows sugar, water follows sugar
Equilibrium
Water stop moving across membranes
Water potential =0
Pure water
Resistance of cell wall to deformation
Rigidity of plant cell wall to prevent the osmotic lysis by hydrostatic pressure (turgor pressure)
Plasmolysis
if water is moving out of the cell
Cell Inside: Hypotonic
Cell outside: hypertonic
Plasmolyzed
Water is moving outside of the cell
Solute potential outside of cell < solute potential cell inside
Cell inside: isotonic
Cell outside: isotonic
Flaccid
Solute potential cell outside is equal to solute potential cell inside
Cell inside: hypertonic
Cell outside: hypotonic
Turgid
Water moving into the cell
Solute potential cell outside > solute potential cell inside
Turgor pressure (osmotic pressure)
pressure from fluid within the cell pushing against the cell wall
How do water molecule move across plasma membrane
water channels
How do water molecules go through aquaporins?
Expression of an aquaporin in an Xenopus oocyte accelerates water uptake
Thermodynamic gradients
First response to solute potential gradient
Intermediate response to solute potential gradient
Late response to solute potential gradient
Equilibrium situation
First response to solute potential gradient
Water diffuses from pore orifice
water pulled thru channel by cohesion
Intermediate response to solute potential gradient
Water diffuses from pore orifice
Water pulled thru channel by cohesion
Pressure potential increases in cytoplasm
Late response to solute potential gradient
water diffuses from pore orifice
Water pulled thru channel by cohesion
Pressure potential increases in cytoplasm
Equilibrium situation
Pressure potential increased to balance solute potential
Equilibrium between cells
No NET flux of water across channel
Tetrameric arrangement (tetramer)
each monomer forms a water channel
Phosphorylation and pH
Modify aquaporin channel activity
How to stop osmosis
You need hydrostatic pressure
Diffusion
water movement during transpiration
Transpiration
Evaporation of water mainly through the stomata of leaves
Movement of water in plants (steps)
- Osmosis
- Bulf flow
- Bulk flow
- Diffusion
Diffusion rate is affected by
Area, distance and gradient
Surface tension
Enhancement of intermolecular attractive forces at the surface
Water movement through the leaf
1) Stomata open
2)Water vapor Diffueses from internal air space, down it concentration gradient, into the air
3) Net loss of water vapor causes air-water- interface to recede towards outer microfibrils
3)Establishment of water gradient begins when the air-water-interface touches outer microfibrils
4)H-Bonding/Cohesion transmits tension at surface to bulk water
5) Time dependent buildup of surface tension
6) Water is pulled towards air-water-interface
Analysis of pathway water flow
Apoplasmic (Apoplastic) pathway
Symplasmic (Symplastic) pathway
Transcellular pathway
Apoplasmic (Apoplastic) pathway
Never goes into the plant cell
Water moves through the intercellular spaces (e.g. cell walls) with no entry into cells
No resistance
Symplasmic (symplastic) pathway
First moves into cell through PM
Water moves through the cells via plasmodesmata
Some resistance
Transcellular pathway
Water moves across the plasma membrane
Passes through all of the membranes (vacuole membrane)
Plasmodesmata
microchannel connecting between plant cells through the cell wall, small channel that allows movement through cell walls to other cells
Tracheids
Primitive water-conducting elements
Present in gymnosperms and angiosperms
Cell size species dependent (is small)
Long, thin cells with tapered ends
Walls reinforced with lignin (support)
Gymnosperm
naked seed
Angiosperm
seed is covered
Pit pair
two pits occurring opposite one another in the walls of adjacent tracheid or vessel elements
Vessels
Advanced water-conducting elements
Present only in the angiosperms
One vessel-
Pits
effective combination of primary anf secondary walls
Caviation
a condition where in an air bubble moves into a vessel or tracheids
Embolism
the blocking of a xylem vessel or tracheid by an air bubble or cavity (xylem blocked)
Embolisms spread
from conduit to conduit; a pathway connecting the embolized vessels is shown in yellow
Embolized xylem vessel
No longer hold water
decrease xylem hydraulic conductance
Tensile strength
ability to resist a pulling force of molecules before breaking their bonds
Cohesion theory
the tensile strength of water is high enough to allow water to be pulled through the Treachiary elements
Xylem is vulnerable to
Cavitation (embolism)