Pre-Lab Quiz Enzymes Flashcards
What is a catalyst?
Is something that speeds up the rate of a chemical reaction by lowering the activation energy for the energy
What type of macromolecule are most enzymes?
Protein macromolecules
When referring to chemical reactions (catalyzed or uncatalyzed), what is meant by the term “rate”?
Rates are expressed as the amount of reactant (substrate) consumed per unit time or the amount of product generated per unit time
What is a substrate? What is the substrate in today’s experiment
- A substance on which enzyme get and convert it into a product
- Hydrogen peroxide
Why are we using yeast as the source of catalase in today’s lab?
Because yeast has a nucleus and all the major organelles. It’s a eukaryote and has catalase just like humans do
Why do you need to use eye protection when doing a catalase assay?
Because hydrogen peroxide can be harmful at high concentrations
What chemical reaction is catalyzed by the enzyme catalase? What role does catalase have in metabolism?
2H2O2>2H20 + 02
Protects the cell from oxidative damage
How will the reaction rate be determined in this lab? Why is the pressure probe used?
Measuring the pressure changes in the reaction chamber using a pressure probe. To measure the pressure of the liquids
What is the mechanism whereby enzymes typically affect the rate of a chemical reaction?
The lowering of the activation energy
