Pre analytical phase

Question 1

Branch of pathology which deals with the study of tissues and/or cells macroscopically and microscopically for the diagnosis of disease.

Answer 1

Histopathology

Question 2

the science concerned in preparing and processing tissue specimen for the detection and treatment of tissue abnormalities utilizing specific reagents, chemical and stains.

Answer 2

Histotechnology

Question 3

a laboratory technologist that specializes in the preparation of tissue specimens for a pathologist to examine for diagnostic, research, or teaching purposes.

Answer 3

Histotechnologist

Question 4

Role of Medtech in histopathology

Answer 4

Specimen preservation, labeling, logging, identification
Preparation of specimen
Equipment and reagent maintenance
Record keeping

Question 5

If fixative cannot be added in a timely manner, the specimen should be placed in a

Answer 5

sterile basin and kept moist with sterile saline or wrapped in saline-dampened sponges until the specimen can be properly placed in fixative.

Question 6

collection is usually done in an

Answer 6

operating room or doctor’s office/clinic.

Question 7

Volume of fixative (formalin) to tissue must be ___ times the volume of the specimen.

Answer 7

15- 20

Question 8

the recommended fixative.

Answer 8

10 % neutral buffered formalin

Question 9

must be made available to all staff handling fixatives.

Answer 9

Safety Data Sheets (SDS)

Question 10

Sources of specimen

Answer 10

incisional biopsy
excisional biopsy
autopsy

Question 11

A small piece of lesions (abnormalities in tissue) or tumor which send for diagnosis before final removal of the lesion or the tumor.

Answer 11

incisional biopsy

Question 12

biopsy-Whole of the tumor or lesion send for examination and diagnosis by the pathologist.

Answer 12

excisional biopsy

Question 13

Tissues or organs are send for the study of disease and its course, for the advancement of medicine.

Answer 13

autopsy

Question 14

Types of Histological preparation

Answer 14

Whole mount
Tissue section
Smears

Question 15

These are preparation of entire animal or organism.

Answer 15

Whole mounts preparation

Question 16

Whole mounts preparation should be no more than ____ mm in thickness.

Answer 16

0.2-0.5

Question 17

Example of whole mount preparation

Answer 17

fungus
parasite

Question 18

The majority of the preparations in histology

Answer 18

Tissue section

Question 19

The tissue (tissue sections) is cut in about ______ mm thick and the processed.
_____ microns thick sections are cut on a microtome.
These are then stained and permanently mounted.

Answer 19

3-5
4- 6

Question 20

For cytologic techniques.
Made from blood, bone marrow and other body fluids.

Answer 20

Smears

Question 21

These are immediately fixed in alcohol to preserved the cellular structures then stained.

Answer 21

Smears

Question 22

Example of smear

Answer 22

Fine needle aspiration cytology

Question 23

Record the specimen data on a log book including the

Answer 23

complete name of receiver, date and time.
designate correct accesion number

Question 24

Requisition form informations

Answer 24

Patient name, date of birth, gender

Complete name and address of physician or laboratory requesting the test

Complete name of person receiving the specimen, date and time

Test and tests to be performed

Procedure performed

Specimen site

Date and time of procedure or specimen collection

Clinical history

Question 25

if more than one specimen is collected during a single procedure; each specimen should be individually identified by anatomic site and or specimen type.

Answer 25

Specimen site

Question 26

any additional information relevant or necessary for a specific test to ensure accurate and timely testing and reporting of results, including interpretation if required.

Answer 26

Clinical history

Question 27

Rejection criteria for specimens

Answer 27

Wrong patient information
Imcomplete patient info
Insufficient volume of fixative or inappropriate fixative
Unsuitable container
Unlabeled container

Question 28

Mailing of specimen

All specimens must be recorded on a chain of custody form or log that includes

Answer 28

Dates and time

patient identification

specimen number

specimen description

purpose of specimen delivery to the pathology department

Question 29

Period of retention for Surgical pathology

Wet tissue
Paraffin blocks
Tissue slide
Surgical pathology report
Accession logbook

Answer 29

2 weeks after final report
10 years
10 years
10 years
2 years

Question 30

Period of retention for Cytology

slides (negative or positive)
FNAC slides
Cytology report

Answer 30

5 years
10 years
10 years

Question 31

Period of retention for Non-forensic autopsy

Wet slides
paraffin blocks
tissue slide
autopsy report

Answer 31

3 months after final report
10 years
10 years
10 years

Question 32

Period of retention for forensic autopsy

Wet stock tissue
Body fluids for toxicology
Accession log and gross photograph (negative)
Paraffin blocks, slides, and reports
DNA analysis

Answer 32

1 year
1 year
Indefinitely
Indefinitely
Indefinitely