Pre analytical phase Flashcards
Branch of pathology which deals with the study of tissues and/or cells macroscopically and microscopically for the diagnosis of disease.
Histopathology
the science concerned in preparing and processing tissue specimen for the detection and treatment of tissue abnormalities utilizing specific reagents, chemical and stains.
Histotechnology
a laboratory technologist that specializes in the preparation of tissue specimens for a pathologist to examine for diagnostic, research, or teaching purposes.
Histotechnologist
Role of Medtech in histopathology
Specimen preservation, labeling, logging, identification
Preparation of specimen
Equipment and reagent maintenance
Record keeping
If fixative cannot be added in a timely manner, the specimen should be placed in a
sterile basin and kept moist with sterile saline or wrapped in saline-dampened sponges until the specimen can be properly placed in fixative.
collection is usually done in an
operating room or doctor’s office/clinic.
Volume of fixative (formalin) to tissue must be ___ times the volume of the specimen.
15- 20
the recommended fixative.
10 % neutral buffered formalin
must be made available to all staff handling fixatives.
Safety Data Sheets (SDS)
Sources of specimen
incisional biopsy
excisional biopsy
autopsy
A small piece of lesions (abnormalities in tissue) or tumor which send for diagnosis before final removal of the lesion or the tumor.
incisional biopsy
biopsy-Whole of the tumor or lesion send for examination and diagnosis by the pathologist.
excisional biopsy
Tissues or organs are send for the study of disease and its course, for the advancement of medicine.
autopsy
Types of Histological preparation
Whole mount
Tissue section
Smears
These are preparation of entire animal or organism.
Whole mounts preparation
Whole mounts preparation should be no more than ____ mm in thickness.
0.2-0.5
Example of whole mount preparation
fungus
parasite
The majority of the preparations in histology
Tissue section
The tissue (tissue sections) is cut in about ______ mm thick and the processed.
_____ microns thick sections are cut on a microtome.
These are then stained and permanently mounted.
3-5
4- 6
For cytologic techniques.
Made from blood, bone marrow and other body fluids.
Smears
These are immediately fixed in alcohol to preserved the cellular structures then stained.
Smears
Example of smear
Fine needle aspiration cytology
Record the specimen data on a log book including the
complete name of receiver, date and time.
designate correct accesion number
Requisition form informations
Patient name, date of birth, gender
Complete name and address of physician or laboratory requesting the test
Complete name of person receiving the specimen, date and time
Test and tests to be performed
Procedure performed
Specimen site
Date and time of procedure or specimen collection
Clinical history
