Practicum 1

Question 1

What was used to stain the cheek cells?

Answer 1

Methylene Blue

Question 2

What was used to stain the Cricket Karyotyping Slide?

Answer 2

Geimsa Stain

Question 3

Passive Transport

Answer 3

Requires no Energy Input
Examples:
- Simple Diffusion (O2)
- Facilitated Diffusion (Na+, K+)
Moves with the concentration gradient

Question 4

Active Transport

Answer 4

Requires Energy (ATP)
Example: NA-K Pump
Moves against concentration gradient

Question 5

Osmosis

Answer 5

Movent of water

Question 6

Hypertonic

Answer 6

Outside particle concentration is greater than inside particle concentration, cell shrinks to let water out

Question 7

Hypotonic

Answer 7

Outside particle concentration is greater than inside particle concentration, cell swells to allow water in.

Question 8

Isotonic

Answer 8

Outside and Inside particle concentration are equal

Question 9

Benedict’s Test

Answer 9

Positive: Orange
Negative: Blue

Question 10

Iodine Test

Answer 10

Positive: Black / Purple
Negative: Amber / Yellowish

Question 11

How does inversion work in a microscope?

Answer 11

Refracted light passing through the lens is “reversed” or inverted when we view it.

Question 12

Phenol / Chloroform

Answer 12

Partially Water Soluble

Protein Solvent

Question 13

SDS

Answer 13

Homogenized cells to chemically lyse them

Disrupts the lipid membranes and denatures the proteins

Question 14

Colchicine

Answer 14

Inhibits polymerization of microtubules, preventing spindle formation necessary for Meiotic cell division.

Question 15

Tripsin

Answer 15

Digests proteins