practicles

Question 1

Unit 2 Lo2

Gel Electrophoresis Steps:

Answer 1

1. DNA fragment are placed into wells
2. Buffer solution is added(to complete the circuit)
3. A cathode is placed near the wells and a anode is place at the other end of the gel.
4. An electric current is applied (has to be direct current)
5. dye/ radioactivity used to visualise DNA

Question 2

Unit 2 Lo2

Chromatography:

Answer 2

1. Set up test tube with solvent at the bottom
2. Take chromatography paper and draw a line in pencil
   2cm from bottom(origin)
3. Cut a disc from leaf with a cork borer (avoid midrib)
4. Place disc onto paper at the center of the line. Crush with glass rod until a stain is left.
5. Put paper into the test tube. put into the rack and let solvent run.
6. Remove paper from test tube when solvent stops near the top and immediately draw line with pencil(solvent front)
7. The paper now has colour spots this is a chromatogram. then calculate the Rf values of each pigment spot .