practice questions

Question 1

Question: Is a brightfield microscope used for qualitative or quantitative observations?

Answer 1

Answer: qualitative

Question 2

Question: Why aren’t light microscopes capable of resolving capabilities similar to electron microscopes?

Answer 2

Answer: Diffraction. All microscopes are considered either “diffraction limited” or “not diffraction limited” (or “super resolution microscopy”)
PALM super resolution microscope - not governed by Abbe’s

Question 3

Question: How can we enhance contrast in a microscope?

Answer 3

Answer: Colorimetric - dyes that can be detected using visible spectrum of light (i.e hematoxylin and eosin)
Fluorochromes - fluorescent probes (have much more utility)

Question 4

Question: Who is Craig Venter?

Answer 4

Answer: He was the one who created an artificial cell capable of replicating
Sped up human genome project

Question 5

Question: Which microscopes have the technology to view living tissues?

Answer 5

Answer:

Fluorescence, Phase, Nomarski, Confocal

Question 6

Question: What is GFP?

Answer 6

Answer:

A fluorescent protein/reporter molecule that is used to do FRET