practical's

Question 1

in the mitosis practical why is
- Hydrochloric acid
- A stain
- Mounted needle
- root tip
needed ?

Answer 1

• hydrochloric acid - softens and loosens the root tip
• stain - it dyes the chromosomes to make them visible
• mounted needle - To lower the coverslip preventing air bubbles
• root tip - where the region of growth is so that is where mitosis occurs

Question 2

Why do we need to squish the root tip in the mitosis practical

Answer 2

So you can get a thin layer of cells so light can pass through

Question 3

What are some of the hazards in the mitosis practical

Answer 3

-scapple - cutting yourself while using it - cut on top of a white tile to prevent slipping

• hydrochloric acid - irritant - wear safety goggles

Question 4

How do you work out the mitotic index

what do you do to make sure your cell count is accurate

Answer 4

number of cells going trough mitosis / total number of cells
x100

examine a large number of fields of view or many cells

Question 5

how do you convert mm into um and vice-versa

Answer 5

mm into um = x1000
um into mm = /1000

Question 6

What is an equation for volume of a new solution by a dilution series

Answer 6

c1xv1= c2xv2
conc of stock solution x volume of stock solution = conc of new solution x volume of new solution