Practical Questions

Question 1

What substance is often used as a model for studying NADPH?

Answer 1

DCPIP is used instead of NADPH

This is because:

1. it is stable, unlike NADPH, and
2. it is blue, so the blue&raquo_space;> colourless reaction can be seen, unlike with colourless NADPH

Question 2

What must you remember to do when finding the concentration of a single substance in a mix of many?

Answer 2

> Add all volumes of all substances together to find the total volume, and
Convert this to litres to find the concentration per litre

Question 3

What is the theoretical concentration of a substance where A = Ɛ ?

Answer 3

Theoretically, this would mean c = 1M.

This can be used to work out the actual concentration.

When A = 1, conc = 1 // Ɛ

Question 4

How many molecules are in a mole?

Answer 4

6 × 10 ^(23)

Question 5

Why would the value of reductive action of chlorophyll molecules be HIGHER in vivo than in vitro?

Answer 5

In a lab experiment, some photosynthetic machinery is damaged whilst preparing the samples.