Metabolism - Block 5 Flashcards
Is Glycolysis + Krebs Cycle an anabolic or catabolic process?
Catabolic: sugar»_space; CO2
Are protein, fat & polysaccharide synthesis anabolic or catabolic processes?
Anabolic: building molecules
Is ATP made or used up during catabolism?
ATP is made
Is ATP made or used up during anabolism?
ATP is used up
Is NAD(P)H made or used up during catabolism?
NAD(P)H is made
Is NAD(P)H made or used up during anabolism?
NAD(P)H is used up
NADPH is “nature’s favourite” what?
Nature’s favourite reducing agent
What is often used as a model for NADPH, and why?
DCPIP is used instead of NADPH
This is because:
1. it is stable, unlike NADPH, and
2. it is blue, so the blue»_space;> colourless reaction can be seen, unlike with colourless NADPH
Where in the cell does glycolysis take place?
The cytosol
Where in the cell does the Krebs cycle take place?
The mitochondrion
What occurs in the first step of glycolysis?
Glucose is converted (phosphorylated) to G6P
What happens to G6P in glycolysis (step 2)?
G6P is converted to F6P (isomerisation)
Which enzyme converts glucose to G6P?
Hexokinase
Which enzyme converts G6P to F6P?
Phospho-hexose isomerase
What happens to F6P in glycolysis (step 3)?
F6P is converted to F(1,6)BP (isomerisation)
Which enzyme converts F6P to F(1,6)BP?
Phosphofructokinase
What happens to F(1,6)BP in glycolysis (step 4)?
It is split into GAP and DHAP (3-Carbon molecules)
Which enzyme converts F(1,6)BP to GAP and DHAP?
Aldolase
What happens to GAP and DHAP in glycolysis (step 5)?
DHAP is converted to GAP, so two GAP are present
What happens to the two GAP in glycolysis?
They are oxidised to GBP (a carboxilic acid with two phosphates)
What oxidant is needed to convert GAP to G(1,3)BP?
NAD+
What important process occurs when G(1,3)BP is converted to G3P?
The formation of (2) ATP (as there are two GBP molecules to begin with)
What happens to G3P in glycolysis?
It is isomerised to G2P
Which steps in glycolysis use ATP?
- The phosphorylation of glucose to G6P
2. The phosphorylation of F6P to F(1,6)BP