Practical biotechnology: embryo manipulations (EMT) and cloning Flashcards
Which is the correct order regarding in vitro embryo production?
Oocyte recovery, in vitro maturation, in vitro fertilization, in vitro culture, embryo transfer
Which out of the following statements is true regarding sex sorted semen preparation?
The process does not impair the spermatozoa cells.
Efficacy is 100% in getting the desired male or female offspring.
The device for this process is usually used 24 hours a day for maximal utilization.
Higher pregnancy rates are achieved by this method, compared to AI with non-sorted semen.
Approx. 50% of the spermatozoa cells are lost during the preparation of sex sorted semen.
The device for this process is usually used 24 hours a day for maximal utilization.
Which out of the following statements is false regarding in vitro embryo production?
The oocytes collected from the donors are immature, that is why in vitro maturation is necessary.
In vitro embryo production is a special method for MOET.
Fertilization can be maintained by conventional IVF or by ICSI.
Embryos require proper culture media and proper environment during in vitro culture.
Oocytes of valuable donors can be recovered surgically, while ovaries of slaughtered animals can be used for research purposes.
In vitro embryo production is a special method for MOET.
Which out of the following statements is false regarding cloning by somatic cell nuclear transfer?
Cloning by differentiated embroynic cell and adult somatic cell nuclear transfer has already been successfull.
Reproductive cloning creates a new multicellular organism, genetically identical to the nucleus donor.
Cloning by adult somatic cell nuclear transfer has not been successfull in mammals; though nuclear transfer of embryonic cells has resulted in viable individuals.
Individuals created by cloning are usually fertile.
Therapeutic cloning means harvesting „personalized” stem cells that can be used to study human development and to potentially treat diseases.
Cloning by adult somatic cell nuclear transfer has not been successfull in mammals; though nuclear transfer of embryonic cells has resulted in viable individuals.
Which out of the following methods cannot be used for increasing the number of female offspring of a valuable cow?
Artificial insemination
Which out of the following statements is false regarding chimeras?
A chimera is originatied from two (or more) different populations of genetically distinct cells.
Embryo splitting is a method for producing chimeras.
Embryo aggregation is a method for producing chimeras.
Chimeras are formed from four (or more) parent cells.
Embryonic stem cell injection into blastocysts is a method for producing chimeras.
Embryo splitting is a method for producing chimeras.
Which out of the following gametes or embryos can be efficiently cryopreserved?
Bull semen, bovine embryos, sex-sorted bovine embryos.
Which out of the following statements is false regarding transgenesis?
A transgenic animal is an organism which have a new gene originated from another species, and the gene was transmitted by crossing two different specieses.
Transgenesis is the process of introducing a transgene into a living organism so that the organism will exhibit a new property and transmit that property to its offspring.
A transgenic animal is an organism which have inserted DNA that originated in a different organism/species.
A transgene is a gene that has been transferred by genetic engineering techniques from one organism/species to another.
A transgenic animal is an organism which have a new gene originated from another species, and the gene was transmitted by crossing two different specieses.
