practical 3 elisa Flashcards
1
Q
a capturing antibody is absorbed onto a solid phase
A
an antigen is then added usually the unknown e.g. viral antigen in serum, transporter protein
2
Q
a labelled detection antibody is then added, if the antigen is present the antibody will bind
A
the substrate is added. the HRP label catalyses the oxidation of the substrate - producing a colour change. the concentration of antigen is proportional to the amount of colour produced
3
Q
we use horse radish peroxidase (TMB) as
A
elisa substrate
other options - alkaline phosphatase labelled antibody, urease, gold nanoparticles, fluorophones, biotin
