practical 2 western

Question 1

2 adipocyte cell lines

Answer 1

3T3-L1 (wild type)
3T3-L1D (CRISPR/Cas9 disrupted)
C1 - cytosol from 3T3-L1, no insulin
C2 - cytosol from 3T3-L1D, no insulin
C3 - cytosol from 3T3-L1, insulin treated
C4 - cytosol from 3T3-L1D, insulin treated
P1 - plasma membrane from 3T3-L1, no insulin
P2 - Plasma membrane from 3T3-L1D, no insulin
P3 - Plasma membrane from 3T3-L1, insulin treated
P4 - Plasma membrane from 3T3-L1D, insulin treated

Question 2

aim of practical

Answer 2

To determine i the protein (GLUT4) translocates on insulin challenge in ‘wild-type’ 3T3-L1 adipocytes and in 3T3-L1 adipocytes that have had the insulin signaling system
disrupted.