Practical 2 test Flashcards
General morphology of trypanosomes
What are the monomorphic types?
T. vivax, T. congolense
General morphology of trypanosomes
What are the pleomorphic types
T. brucei
General morphology of trypanosomes
What are the forms of trypanosomes
- Trypomastigote (metacyclic form - infective stage),
- epimastigote,
- promastigote,
- amastigote
Transmission of trypanosomes can be ?
- cyclic
- non-cyclic
Transmission of trypanosomes
CYCLIC
An arthropod vector is a necessary biological vector in which they multiply and infective stage develops e.g. T. brucei – Glossina spp.
Transmission of trypanosomes
NON-CYCLIC
Only mechanical transmission by an arthropod vector e.g. T. evansi – S. calcitrans
Other name of DOURINE
What is it a genetic variant of?
Dourine – “Trypanosoma equiperdum”
(genetic variants of T. brucei evansi and T. brucei equiperdum)
- Which form of giardia is found in the feces
(both trophozoites and cysts, I believe)
- Which of the following cause necrosis of the liver
(Trichomonas gallinae and Histomonas meleagridis)
- Which of the following is the vector for Leishmania
(Sandflies, but he said he wouldn’t count this question because it wasn’t in the practical handout)
- Which of the following is a venereal disease
(dourine)
- which parasite has 4 flagella?
(trichomonas gallinea)
- which parasite is not transmitted via vector
(giardia)
- where does histomonas melgritis causing ameobid form?
in the liver
- which parasite can infect equines and donkeys?
Dourine (Trypanosoma equiperdum)
TRYPANOSMOSIS
Which are Monomorphic
T.Vivax and T.Congolense
TRYPANOSMOSIS
Which are Pleomorphic
T. Brucei
TRYPANOSMOSIS
What are the forms?
- Trypomastigote (metacyclic, infective)
- Epimastigote
- Promastigote
- Amastigote
Transmission of Trypanosomes can either be? or ?
Cyclic or non cyclic
CYCLIC Transmission of Trypanosomes
Types?
An arthropod vector is nessesary
T. Brucei - Glossins ssp
NON-CYCLIC Transmission of Trypanosomes
Types?
Only mechanical transmission by an arthropod vector.
T.Evansi - S.calcitrans
What are the causative agent of DOURINE?
Genetic variants of
T. Brucei Evansi and T. Brucei Equiperdum
Clinical signs of DOURINE
-
GENITALS
- inflammation and ulceration, oedema (ventral abdominal too), hypopigmentation of skin
-
SKIN
- – “dollar spots”, urticaria (2-5 cm in diameter) on withers, thorax, neck, etc.; they are neither warm nor painfull
-
NERVES
- – Hyperaesthesia, anaesthesia, ascending motor paralysis
In dourine - What do we examine during the parasitological diagnosis?
In parasitaemia - Motile Trypanosomes (TRYPOMASTIGOTES)
- Fresh film of blood
- Centrifugation: Microhematocrit may help to find them
GIEMSA STAINING
Diseases of TRYPANOSOMES/TRYPANOSOMOSIS
- Dourine
- Nagana
- Surra
What are the species of NAGANA?
- T. Vivax
- T. Congolense
- T. Brucei Brucei
What are the clinical signs and species of NAGANA in Horses
T. Brucei Brucei
Oedema of the limbs and genitalia
(General: enlarged lymph + spleen, anaemia, cell degeneration+inflamatory infiltrates)
What are the clinical signs and species of NAGANA in Dogs and Cats
T. Brucei and T.Congolense
- Anemia
- Myocarditis
- Ataxia
- Convulsions
(General: Lymph and spleen enlargements, anaemia, cell degeneration and inflammatory infiltrates)
How to detect NAGANA
- Peripheral blood/Ln
- Dark phase contrast microscope - TRYPOMASTIGOTES
- In parasitaemia - motile trypanosomes in fresh blood
- Centrifuge help (microhematocrit- btw buffy coat and plasma)
- Serological methodes
Species responsible for SURRA?
N. Brucei Evansi
How to detect SURRA
Giemsa staining of blood smares - Detection of TRYPOMASTIGOTE
Serology
Species responsible for LEISHMANOSIS
- Leishmania Tropica
- L. Infantum
- L. Donovani
- L. Chagasi
ZONOSIS!!
Clinical signs of LEISHMANOSIS
Forms
Cutan form
Visceral form
Clinical signs of LEISHMANOSIS
CUTAN FORM (species)
More common in dogs
- Alopecia
- Dermatitis
- Ulceration
Clinical signs of LEISHMANOSIS
VISCERAL FORM (species)
In dog
- Intermittent FEVER
-
Enlargement of
- Lymphnodes
- Spleen
- Liver
- Cachexy
- Anaemia
- Diarrhea
- Ataxia
Detection of LEISHMANOSIS
- Demonstration of the AMASTIGOTE form from smares/scrapings
- Biopsy
- Serological methods
- PCR
Species of GIARDIOSIS
Giardia duodenalis
ZONOSIS!!
How to detect GIARDIOSIS
- Direct examination of FECAL SMARE + Phys.saline + Lugol
- Detection of moving TROPHOZOITES (vegitative form)
- Thin fecal smare stained with GIEMSA
- FLOTATION - Concentration of CYSTS (infectious form)
- Immunological methods
How to detect GIARDIOSIS
Morphology of the VEGITATIVE FORM
TROPHOZOITES (Vegitative form)
- Pare shape
- Symmetrical
- 2 nucleus
- 2 Axostyles
- 4 pairs of flagella (8 flagella)
- Flagella movement = Jerky shuffling = like dead leaves in the wind.
How to detect GIARDIOSIS
Morphology of the Infectious form
CONCENTRATION OF CYSTS = Infectious form
- Oval
- 4 nuclei
TRICHOMONOSIS of cattle
Which species
TRICHOMONOSIS FOETUS
TRICHOMONOSIS of cattle
Clinical signs in Bulls
Inapparant signs (not notifiable), rarely inflamation of mucous membr.
URETHRAL DISCHARGE
TRICHOMONOSIS of cattle
Clinical signs in Cows, Heifers
- Vestibulitis
- Vaginitis
- Endometritis
- Pyometra
- Vaginal discharge
- Early abortion
- Anoestrus
TRICHOMONOSIS of cattle
Necropsy findings
Greyish yellow pappules on the chorion of the foetus
TRICHOMONOSIS of Birds
Species
TRICOMONAS GALLINAE
TRICOMONAS GALLINAE
NECROSCOPY FINDINGS
Yellowish membrane in mouth, pharynx, oesophagus, proventriculus, crop
2 forms
- HEPATIC FORM = Necrotic lesions in LIVER
- GENERALIZED FORM = Necrotic lesions in LIVER, HEART & AIRSACS
HISTOMONOSIS
Disease
“Black Head Disease”
Infectious enterohepatitis
Which species are prone to Black head disease?
TURKEY (young)
Chicken
Guinea fowl
phesant
etc
Name the parasite in Black head disease
HISTOMONAS MELEAGRIDIS
Forms of Histomonas/Histomonosis
- Flagelated
- Amoeboid
Where do you find the flagellated form of histomonas?
In Caecal lumen
(round, one flagella)
Where do you find the amoeboid form of histomonas?
In tissues (liver)
(PLEIOMORPHIC)
What are the necroscopy findings of Histomonosis “black head disease”
- Yellowish, necrotic, caseous material in the lumen of CAECUM
- Necrotic Foci with sharp edges in the LIVER, dry cut surface
- Necrotic lesions in the spleen, lung and kidney, wet gut surface of LIVER - GENERALIZED FORM IN GUINEA FOWL
ENTAMOEBOSIS
Zoonotic or not?
Not, its ANTHROPOZOONOSIS
ENTAMOEBOSIS
What to detect/Parasitological diagnosis?
- Fresh faecal smear+Phys.saline and Lugol
- DETECTION OF MOVING TROPHOZOITS (veg)
- FLOTATION - Concentration of CYSTS (inf)
- Immunological methods
NO CYSTS IN FAECES OF ANIMALS