Practical #2

Question 1

Amylase breaks down

Answer 1

Starch into glucose

Question 2

Iodine tests

Answer 2

Detects starch(yellow=negative for starch) (blue=positive for starch )

Question 3

When using a spectrophotometer, you make a tube called a blank (short for blank sample). What should you
put in your blank?

Answer 3

The blank should contain everything in your sample EXCEPT the 1 molecule you are measuring

Question 4

What was tested for Tetrazolium experiment?

Answer 4

Whole cells

Question 5

Tetra experiment
Beans soaked over night:

Answer 5

They had a pink-red color, which is a positive result for the tetrazolium test, indicating
redox reactions are occurring in living cells.

Question 6

Tetra experiment
Autoclaved beans:

Answer 6

They had no color change (remaining white), which is a negative result for the tetrazolium
test, indicating redox reactions were not occurring as they were non-living cells

Question 7

For DPIP experiment what was tested?

Answer 7

mitochondria extracted from cells

Question 8

Succinate is used in what step of cellular respiration?

Answer 8

Citric acid cycle

Question 9

The tube with 0ml of succinate acted as the negative control. Why?

Answer 9

With no succinate (the substrate for this reaction), we expect no reaction to occur. Since
we expect a negative result to occur, this is a negative control for the experiment

Question 10

Alternate hypothesis for DPIP experiment

Answer 10

Increasing succinate concentration will result in DPIP changing color faster