Practical 2 Flashcards
1
Q
Complex 1
A
NADH dehydrogenase P:O= 2.5 RCR= 5-8 substrate :NADH/ glutamate/ malate electron carrier: FMN,FeS H+ movement: 4
2
Q
Complex 2
A
Succinate dehydrogenase P:O= 1.5 RCR= 3-5 substrate :succinate to fumarate electron carrier: FADH,FeS H+ movement: none
3
Q
complex 3
A
Q oxidoreductase P:O= NA RCR= NA substrate :QH2 electron carrier: cyt b, FeS, cyt C1 H+ movement: 4 (from 2x QH2)
4
Q
Complex 4
A
cytochrome oxidase P:O= 0.5 RCR= 1-2 substrate :ascorbate, TMPD reduce cyt c electron carrier: Cu, cyt a, cyt a3 H+ movement: 2
5
Q
Complex 1 product
A
QH2 for complex 3
6
Q
complex 2 product
A
QH2 for complex 3
7
Q
Complex 3 product
A
2e for cyt c, 2 e stays behind in complex 3
8
Q
complex 4 product
A
water
9
Q
complex 1 inhibitor
A
rotenone, irreversibly blocks 2e from FMN to FeS
10
Q
complex 2 inhibitor
A
malonate
11
Q
complex 3 inhibitor
A
antimycin, blocks 2e moving from cyt b1 to cyt bh
12
Q
complex 4 inhibitors
A
cyanide
13
Q
uncouplers
A
2,3 DNP
Oligomycin
14
Q
P:O ratio
A
moles of ADP added/ moles of oxygen consumed in state 3
15
Q
theoretical P:O calculation
A
number of H+ ejected during ETC/ number of H+ used to synthesise each ATP
Can use alternative calculation as assumed that all ADP added is converted to ATP when electron donor substrate is oxidised, and hydrolysis back to ADP is at negligible rate
