Enzyme Kinetics Flashcards
1
Q
Michaelis menten equation
A
Vo= Vmax [S]/ Km + [S]
2
Q
Vmax
A
Max rate at enzyme conc
3
Q
Km
A
Substrate conc where rate is Vmax/2
4
Q
Kcat (turnover number)
A
Kcat= Vmax/ [E]total
5
Q
Km
A
Substrate conc where enzyme works at half its max rate
6
Q
Kd
A
How tightly E and S bind (affinity)
7
Q
Km and affinity relationship
A
Low Km= high affinity
8
Q
Lineweaver burk plot equation
A
1/Vo = (Km/ Vmax) x (1/[S]) + 1/Vmax
9
Q
Competitive inhibition
A
Increases apparent Km without altering V max
More substrate needed to achieve Vmax/2 as inhibitor effects must be overcome
10
Q
Non competitive inhibition
A
Alters V max but not Km,
Binds to both E and ES and reduces catalytic activity
