Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

pathology coursework exam > practical 1- H&E staining > Flashcards

practical 1- H&E staining Flashcards

1
Q

what governs the overall staining pattern

A

rate of which a dye is taken up by a tissue component or the rate of loss of due from a tissue component

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what are the 3 layers composing the skin

A

epidermis dermis subcutis (hypodermis)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

In H&E staining, how do we differentiate a section after staining with harris’s haematoxylin and why do we do this

A

differentiate in 1% acid alcohol for 2-5 seconds use of acidic solutions to remove excess background staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

In H&E staining, what step is taken after dewaxing, hydrating, haematoxylin staining, differentiating and blueing

A

immerse in eosin for 2-4 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

in a rate depending staining process, what is a progressive dye

A

dyes staining more intensely the longer the tissue remains in the dye bath

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what colour stain does eosin produce

A

red cytoplasmic stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

where might you see cells in the process of dividing (mitosis)

A

mucosa- base of intestinal crypts, this is to replenish cells at the epithelium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

In H&E staining, what blueing reagent is used and why do we use it?

A

Scotts solution necessary to convert nuclear colouration from reddish purple to crisp blue/purple enhances the contrast of the H&E stain by increasing the crispness of the haematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what is meant by basophilia

A

tissues with affinity for haematoxylin (basic dye) is base loving

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what is the function of the muscularis externa

A

undergoes segmentation contractions (which mixes chyme in the small intestine) and weak peristaltic contraction (moves chyme along in the small intestine)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what does harris’s haematoxylin use to alter the specificity of its stain explain the procedure

A

uses aluminium salts (haemalum) as a mordant rinse slide in tap water to blue the section haematoxylin used regressively with controlled differentiation in acid alcohol reblueing in tap water (must be alkaline)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

how many layers of cells are there in the absorptive epithelium proper

A

mucosa- one layer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what is the function of the submucosa

A

contains brunners galnds, they secrete alkaline mucus which neutralises gastric acid in chyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

in a rate depending staining process, what is a regressive dye

A

a dye may be selectively lost/removed fro a tissue to distiniguish/differentiate between components with stronger or weaker affinities for the dye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

how is haematoxylin produced

A

extracted in water and precipitated with urea

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what type of dye are the following Ehrilchs, Mayers, Harris, Gills, Coles, Delafield, Carazzis

A

haematoxylin dyes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

what type of a staining procedure is H&E staining

A

regressive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

what is the function of enteroendocrine cells and paneth cells

A

enteroendocrine cells secrete hormones (somatostatin) paneth cells have an immune cell function

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

what is eosin and how does it work

A

acidic dye, but binds to basic (eosinophillic) compounds/structures

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

see the skin diagram in the test booklet, label A-L

A

A- stratium corneum (cornified/horny layer) B- pickle cell layer C- dermis D- fat E- sweat gland F- hair follicle G- blood vessels H- sebaceous (oil) gland I- lymph vessel J- sensory nerve receptor K- hair L- pores

21
Q

In H&E staining, how do we dewax a section

A

place in histoclear for 5 minutes

22
Q

what is meant by acidophilia

A

tissues with affinity for eosin (acidic dye) is acid loving

23
Q

look at tissue A in the test booklet, label A, B and C how are the columns of hepatocytes that radiate from the central vein seperated

A

A- portal triad (proper hepatic artery, hepatic portal vein and bile duct) B- hepatic vein C- hexagonal hepatic lobule (bile canaliculi, sinusoidal capillaries, hepatocytes) seperated by canniculae of the bile ducts

24
Q

In H&E staining, what do we do after dewaxing and hydrating a sample

A

immerse in Harris’s haematoxylin for 5 minutes

25
Q

what alters specificity of a haematoxylin stain

A

use of a mordant (inorganic stain-fixing solution)

26
Q

what is the most common method used to enhance contrast between cell structures and tissues

A

dyes e.g. Haematoxylin and Eosin staining

27
Q

where does haematoxylin come from

A

natural product made from haematoxylum campechianum (logwood)

28
Q

what are the 3 zones in the duodenum state what each zone consists of

A

mucosa- inner absorptive epithelium with associated connective tissue (such as blood vessels) consists of lamina propria and muscularis mucosa submucosa- underlying zone of loos CT muscularis externa- outer muscle coat, consists of circular and longitudinal layers

29
Q

what is meant by eosinophilia

A

eosin loving, dye term used to describe tissue affinity

30
Q

what colour staining does H&E produce

A

red/blue

31
Q

what procedure does H&E staining rely on

A

acid-base dye interactions

32
Q

what is the function and structure of the subcutis (hypodermis)

A

layer containing main vascular supply contains adipose tissue and fibrous tissue

33
Q

what is the function and structure of the epidermis name the layers, inner to outer

A

upper, protective zone comprising several sublayers of stratified squamous epithelium boys smell girls like chocolate basale, spinosum, granulosum, corneum

34
Q

what are the different types of eosin stains

A

eosin Y and eosin B

35
Q

In H&E staining, how do we dehydrate a sample

A

by passing through alcohols of increasing concentration 70% alcohol for 1 minute 90% alcohol for 1 minute absolution (100%) alcohol for 1 minute

36
Q

what is the function and structure of the dermis

A

underlying zone containing several glands and structures which include: hair follicles, sebaceous glands, eccrine sweat glands and apocrine glands

37
Q

look at the diagram of tissue C- duodenum on the test booklet and label A-G

A

A- epithelial layer of cells B- villus C- lamina propria D- muscularis mucosae (mucosa) E- intestinal crypts (crypt of lieberkuhn) F- brunners gland G- serosa (outer layer)

38
Q

how do goblet cells and enterocytes differ

A

goblet cells are cup shaped (involution doesnt stain)- secrete mucus enterocytes are rectangular and have an elongated nuclei

39
Q

what is meant by dye-tissue affinity in H&E staining

A

measure of a dye to transfer from a dye bath onto a tissue section

40
Q

how is the muscularis externa arranged

A

outer thinner layer has longitudinal arrangement inner thicker layer has circular arrangement

41
Q

put the following duodenum layers in order (inner to outer) lamina propria sub mucosa adventitia epithelium muscularis propria muscularis mucosa

A

epithelium lamina propria muscularis mucosa sub mucosa muscularis propria adventitia

42
Q

name 3 other methods used to enhance contrast between cell structures and tissues

A

polarised light, dark field, phase contrast

43
Q

how thin must tissue sections be when viewing through a light microscope

A

<15µm

44
Q

what process must a haematoxylin dye undergo before use as a stain

A

requires oxidation to haematein

45
Q

In H&E staining, how is the sample cleared at the end

A

immersed in histoclear for 5 minutes and mounted in DePeX

46
Q

what does a haematoxylin dye stain, give examples

A

basophillic structures such as nucleic acids, ribosomes

47
Q

In H&E staining, how do we hydrate a sample

A

by passing through alcohols of decreasing concentration 100% (absolute) alcohol for 1 minute 90% alcohol for 1 minute 70% alcohol for 1 minute

48
Q

what is eosin derived from

A

fluorescein (fluoresces)

pathology coursework exam (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions