Practical 1 Flashcards
LAB BIO II
parts of a microscope image
A- Ocular
C-Brightness control Know
D- Head
F- objective lense
J-stage
M-Arm
N- cors focus knob
O- fine focus knob
Both the ___ and the _____ help magnify your view on a microscope.
Oculars and Objective Lenses
How much magnification do oculars provide?
10x
What 3 magnifications do objectives provide?
4x 10x 40x
How do you calculate total magnification
whats the total magnification of 4x?
multiply number on objective by 10
40x
As the magnification of the objective lenses ______, the field of view or diameter of field______.
increases, decreases
As the magnification _____, the light intensity through the ocular lenses______.
increases, decreases
As the magnification _____, the working distance, or the distance between the objective lens and the slide, _____.
increases, decreases
summarize how to use a micropippette
select the corect pippette
add a pippette tip
push down to the first stop to suck up the liquid
push down to the second stop the release the liquid
release the pippette tip
Pippette P20 (2-20 ul)
Minimum ex. Maximum
0. 1. 2
2. 2. 0
0. 5. 0
2ul. 12.5ul. 20ul
Pippette P200 (20-200)
Minimum ex. Maximum
0. 1. 2
2. 2. 0
0. 5. 0
20ul. 125ul. 200ul
Pippette P100 (10-100)
Minimum ex. Maximum
0. 0. 1
1. 7. 0
0. 5. 0
10ul. 75ul. 100ul
Pippette P1000 (100-2000 ul)
Minimum ex. Maximum
0. 0. 1
1. 7. 0
0. 5. 0
100ul. 750ul. 1000ul
how to calculate accuracy?
actual value- measure value
—————————————- = accuarcy %
acttual value x 100
lab reports are written in the ___ person and the ____ tense.
3rd, past
Prokaryotes vs eukaryotes
Pro: smaller, simpler, no nucleus (nucleiod region), no membrane bound organelles, binary fisson for replication.(DNA replicates and the cell splits)
Euk: larger, complex, nucleus, membrane bound organelles, numerous methods of replication
3 domains of life and what kind of cell they are
Eukarya- eukaryotic
Archea- prokaryotic
Bacteria- cyanobacteria- prokaryotic
Bacteria celll shapes
coccus
sprillia
bacillius
Groups of bacteria and their characteristics/importance
charateristics: peptidoglycan cell walls, cell membrane with a phosphlipid bilayer
importance: human disease, cyanobacteria first to produce oxygen
Gram Staining:
What feature of Bacteria is stained
Expected results of Gram Staining
Why does Gram Staining not work for Archaea?
The pebtidoglycan in cell walls
If the bacteria is gram negative it will be pink, if it is gram positive it will be purple.
Archa has pseudopetidoglycan in its cell walls not petidoglycan.
Aseptic techniques
gloves, sterile equiptment, flow hood, don’t talk to samples, don’t leave plates open.