post translational workshop

Question 1

what does BLAST stand for ?

Answer 1

basic local alignment search tool

Question 2

what is BLAST used for ?

Answer 2

to compare nucleotides or protein sequences to sequence databases and this calculates the statistical significance

Question 3

what can BLAST identify ?

Answer 3

the members of a gene family

Question 4

if E is 0 in BLAST what does this mean ?

Answer 4

there is a high likely hood that the protein is related

Question 5

what is EXPASY used to identify ?

Answer 5

a proteins molecular weight mW and it’s isoelectric point

Question 6

units used for mW ?

Answer 6

Da
kDa
g/mol
g/mol-1

Question 7

compbio dundee function ?

Answer 7

it is used to predict secondary structures like an alpha helix or beta sheet

Question 8

what is the function of spoctupus ?

Answer 8

this allows us to predict the signal sequence for import of a protein into the ER

Question 9

is the predicted mW of a mature protein lower or higher after ER import ?

Answer 9

lower

Question 10

is the N terminal amino acid always methionine ?

Answer 10

not necessarily

Question 11

can a protein with a signal sequence be found at the cytosol ?

Answer 11

nope

Question 12

does the signal protein stay attached after ER import ?

Answer 12

nope

Question 13

what happens if the protein lacks a transmembrane helix ?

Answer 13

it can be secreted

Question 14

what needs to happen for an amino acid to be phosphorylated ?

Answer 14

it needs to be exposed to the cytosolic kinases , so they need to be hydrophilic.

Question 15

does phosphorylation occur at the N or C terminus ?

Answer 15

C

Question 16

for prenylation what is the attachment site ?

Answer 16

the cysteine

Question 17

what terminus serves as a signal for a prenylation ?

Answer 17

the NH2 terminus

Question 18

for prenylation what does the COO2 terminus serve as ?

Answer 18

a signal for the GPI anchor

Question 19

where does the myristoylation occur ?

Answer 19

at the N terminus