Post Lab Extended Ver: Enzyme Factors Flashcards
Factors Affecting Enzyme Activity
Environmental Conditions
❏ Temperature
❏ pH
❏ Substrate concentration
❏ Enzyme Concentration
is the temperature at which enzymatic reaction
occur fastest
Optimum temperature
For most enzymes the optimum temperature is about
40°C
Most enzymes however are fully denatured at
70°C
What temp possible of renaturation?
Cold Temp
This change in ionization will affect the binding of
the substrate with the active site.
pH
Salivary amylase has an optimum pH of
6.8 pH
Salivary amylase denature in pH of
pH 9.0
Pepsin has an optimum pH of
2.0
Pepsin denature in pH of
pH 4.0
Arginase has an optimum pH of
pH 9.5
Arginase denature in pH of
pH 11
True or False
The rate of reaction increases as substrate
concentration increases
True
Maximum activity occurs when the enzyme
is
Saturated
Maximum activity occurs when the enzyme
is saturated. This is called _____
saturation point
