Post Lab Extended Ver: Enzyme Factors Flashcards
Factors Affecting Enzyme Activity
Environmental Conditions
❏ Temperature
❏ pH
❏ Substrate concentration
❏ Enzyme Concentration
is the temperature at which enzymatic reaction
occur fastest
Optimum temperature
For most enzymes the optimum temperature is about
40°C
Most enzymes however are fully denatured at
70°C
What temp possible of renaturation?
Cold Temp
This change in ionization will affect the binding of
the substrate with the active site.
pH
Salivary amylase has an optimum pH of
6.8 pH
Salivary amylase denature in pH of
pH 9.0
Pepsin has an optimum pH of
2.0
Pepsin denature in pH of
pH 4.0
Arginase has an optimum pH of
pH 9.5
Arginase denature in pH of
pH 11
True or False
The rate of reaction increases as substrate
concentration increases
True
Maximum activity occurs when the enzyme
is
Saturated
Maximum activity occurs when the enzyme
is saturated. This is called _____
saturation point
An additional non-protein molecule that is
needed by some enzymes necessary for
proper functioning. Their main role is to
assist.
Cofactor
Tightly bound cofactors to the apoenzyme
are called
prosthetic groups
Cofactors that are bound and released
easily to the apoenzyme are called
coenzymes
❏ Substances that reduce the rate of enzymatic reactions.
Inhibitor
These compete with the substrate molecules
for the active site.
Competitive Inhibition
Distorts the shape of the enzyme when it is
bound to it, thus enzyme can’t bind the
substrate
Noncompetitive Inhibition
Is a process in which activation or
inhibition of the first reaction in a
reaction sequence is controlled by
a product of the reaction sequence
Feedback Control
