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histo lab > post lab 1-3 > Flashcards

post lab 1-3 Flashcards

1
Q

It supports the tube and connects it to the base

A

Arm

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2
Q

The bottom of the microscope, used for support

A

Base

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3
Q

It is where you look to see the image of your specimen

A

Eyepiece

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4
Q

Instrument used to see objects that are too small to the naked eye

A

Microscope

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5
Q

It connects the eyepiece to the objective lenses

A

Body tube

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6
Q

This is the part that holds two or more objective than says and can be rotated easily change power

A

Revolving nose piece

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7
Q

The flat platform where you place your slide

A

Stage

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8
Q

Small, round knob of the side of the microscope used to fine-tune the focus of your specimen

A

fine adjustment knob

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9
Q

This is usually used specifically using HPO

A

Fine adjustment knob

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10
Q

Large, round knob on the side of microscope used for focusing the specimen

A

coarse adjustment knob

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11
Q

Holds the slide in place

A

Stage clips

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12
Q

It is the hole in the stage through which the base light reaches the stage

A

Aperture

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13
Q

It is used to call it and focus the light from the illuminator onto the specimen

A

Condenser

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14
Q

It is located under the stage often in conjunction with an iris diaphragm

A

Condenser

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15
Q

It controls the light going thourpugh aperture

A

iris diaphragm

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16
Q

It is used to reflect light to the specimen for source of light

A

Mirror or light source

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17
Q

It is used to increase the magnification of the specimen

A

Objective lenses

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18
Q

Total magnification of scanning

A

40

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19
Q

Total magnification of LPO

A

100

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20
Q

Total magnification of HPO

A

400

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21
Q

Total magnification of OIO

A

1000

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22
Q

Magnification lenses of 40 times

A

High power objective

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23
Q

Magnification of scanning

A

Four times

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24
Q

Magnification lens of LPO

A

10

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25
Q

Magnification length of white

A

100

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26
Q

t or f: Always Observe the specimen or object using the high power object first

A

false, low power

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27
Q

It is to bring the object into focus

A

Coarse adjustment knob

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28
Q

Bring the object into sharp focus

A

Fine adjustment knob

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29
Q

t or f: Use only the final adjustment knob when using the highest power objective

A

true

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30
Q

Keep one eyes open to reduce eyestrain

A

False, both eyes

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31
Q

Keep eye slightly below the eyepiece to reduce eyelash interference

A

false, above

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32
Q

To find out the total magnification of the object, multiplied the power of the eyepiece lens by the power of the objective

A

true

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33
Q

t or f: Always use one hand to move the microscope

A

false, both hands

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34
Q

t or f: Place one hand around the arm and then put your other hand under the base of the scope for support

A

True

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35
Q

t or f: One nucleus in one sense except for WBC and platelets

A

false: RBC and platelets

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36
Q

t or f: Muscle cells are non-nucleated

A

false: Multi nucleated

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37
Q

Type of cells that contains a nucleus and organelles

A

Eukaryotic cell

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38
Q

A type of cell that does not include the nucleus or specialized organelles

A

Prokaryotic cell

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39
Q

It make up the living structure of the body

A

Somatic cell

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40
Q

These are germ cells that involved in sexual reproduction

A

Sex cells

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41
Q

It is a double membrane bound control center of the cell

A

Nucleus

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42
Q

These are hereditary units

A

genes

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43
Q

It is a nuclear structure that has double membrane that separates the nucleus from the cytoplasm

A

Nuclear envelope

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44
Q

It is a nuclear structure that has numerous openings in the nuclear envelope

A

Nuclear pores

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45
Q

It is a nuclear structures that control the movement of the substance between nucleus and cytoplasm

A

nuclear pore

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46
Q

It is a nuclear structure that is a small discrete spherical densely staining structures made up of RNA and produces ribosomes

A

nucleolus

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47
Q

These are long molecules of the Deegan is that combines with protein molecules

A

Chromosomes

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48
Q

protein molecules in the DNA

A

Histones

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49
Q

It is very small rounded bodies found on both ends of the nucleus

A

Centrosome

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50
Q

It regulates the rate of cell division and multiplication

A

Centrosome

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51
Q

It consist of cylinder with a microtubules arrange peripherally in a circle

A

Centrioles

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52
Q

Two types of hereditary traits

A

Phenotype and genotype

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53
Q

these are physical type of traits that is observable aspects of the hereditary handed down by parents of the offspring

A

Phenotype

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54
Q

This type of trait is non-physical and non-observable

A

Genotype

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55
Q

Center of chromosomes

A

Centromere

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56
Q

It is the arrangement of molecule with in the membrane that resembles the sea of lipids

A

Fluid Mosaic model

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57
Q

These are the gatekeepers to certain molecules and ions

A

protein

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58
Q

Organelle that separates the cells internal environment from the outside Environment

A

Plasma membrane

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59
Q

Composition of plasma membrane

A

Carbohydrates proteins and lipids

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60
Q

It is a small is spherical sac formed by budding off from a membrane

A

Vesicle

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61
Q

Materials move into a sale in a vesicle formed from the plasma membrane

A

Endocytosis

62
Q

Vesicles fuse with the plasma membrane’s, releasing their contents into the extracellular fluid

A

Exocytosis

63
Q

It is a transport of the combination of endocytosis and exocytosis

A

Transcytosis

64
Q

It is an intracellular fluid that surrounds the organelles And a marker of lactate the hydrogenase

A

cytosol

65
Q

1 g of carbohydrates is equal to how many calories

A

4 cal

66
Q

Heat insulator and tissue elasticity

A

Fat or lipids

67
Q

Fat or lipids are stored in

A

Triglyceride

68
Q

It is for tissue building

A

Protein

69
Q

Cytoplasm composition

A

Carbohydrates, fats or lipids, proteins, electrolyte

70
Q

Most abundant composition of cytoplasm

A

Water

71
Q

Two types of water in cytoplasm

A

Fix water and free state water

72
Q

water composition in males

A

55 to 65%

73
Q

Water composition of females

A

45 to 55%

74
Q

Water composition in infants

A

70 to 80%

75
Q

It is a network of fibers extending throughout the cytoplasm

A

Cytoskeleton

76
Q

Also forms the major component of cilia and flagella

A

Cytoskeleton

77
Q

Cytoskeleton consist of

A

Microfilaments, microtubules and Intermediate filament

78
Q

it is a part of cytoskeleton that helps to cell change and maintain its shape

A

Microfilament

79
Q

It is a part of cytoskeleton that often used by the cells to hold their shape

A

Microtubules

80
Q

Site for proteins synthesis

A

Ribosomes

81
Q

It translates the genetic code into polypeptide chains

A

Ribosomes

82
Q

It is found attached to the Rough endoplasmic reticulum or free in the cytoplasm

A

Ribosomes

83
Q

It is a process where amino acid join together by the peptide bond or polypeptide chains

A

Protein synthesis

84
Q

Composition of ribosome

A

60% RNA 40% protein

85
Q

These are specialized and metabolically active structures or little organs within the cells

A

Organelles

86
Q

It’s transport of materials within the cell

A

Endoplasmic reticulum

87
Q

It is a network of continuous sacs, studded with ribosomes

A

Rough endoplasmic reticulum

88
Q

It manufactures, process and transport the proteins for export from the cell

A

Rough endoplasmic reticulum

89
Q

It involved in the synthesis of lipids, carbohydrate metabolism and detoxification of drugs and poisons

A

SER

90
Q

Golgi complex is consist of how many flattened membranous sacs

A

3 to 20

91
Q

it’s temporary store depot for cellular secretion and site of synthesis of large carbohydrates

A

Golgi complex

92
Q

It is an intracellular defense mechanism that will digest the substance digested by the phagocytosis

A

Lysosome

93
Q

It is a single membrane bound structure that contains digestive enzyme

A

Lysosome

94
Q

It does not engage in any digestive activity and newly formed lysosomes

A

Primary lysosomes

95
Q

Sites of current or past digestive activity

A

Lysosomes secondary

96
Q

It is where the production and degree Dacian of hydrogen peroxide occurs

A

Peroxisome

97
Q

These are the series of folds in the mitochondria

A

cristae

98
Q

These are the large central fluid filled cavity of mitochondria

A

Matrix

99
Q

These are the cytoplasmic inclusion

A

Vacuoles, inclusion, pigment and fat droplets

100
Q

It is a color compound that says my produce oranges

A

Pigments

101
Q

Color of the bilirubin

A

Yellow

102
Q

Color of biliverdin

A

green

103
Q

It is the process by which we give color to a section

A

Staining

104
Q

It is used to identify a differentiate bacteria

A

Gram stain

105
Q

Gram-positive color

A

Purple or violet

106
Q

Gram-negative color

A

Red or pink

107
Q

Bacteria in gram positive

A

staphylococci and streptococci

108
Q

Bacteria in gram negative

A

neisseria

109
Q

Components of giemsa stain

A

Methylene blue and eosin

110
Q

It is used to identify acid-fast bacilli

A

ziehl nielsen stain

111
Q

primary stain in hot sputum

A

carbol fuschin

112
Q

primary stain in cold tissue

A

carbol fuschin

113
Q

secondary stain in cold tissue

A

malachite green

114
Q

secondary stain in hot sputum

A

methylene blue

115
Q

hot sputum is by

A

ziehl nielsen

116
Q

cold tissue by

A

kinyoun

117
Q

steps in gram staining ( in order)

A

crystal violet, iodine, alcohol and safranin

118
Q

mordant in hot sputum

A

physical (steam)

119
Q

mordant in cold tissue

A

chemical (tergitol)

120
Q

decolorizer in hot sputum

A

0.05 or 0.1 N hcl acid in 70% ethanol

121
Q

decolorizer in cold tissue

A

0.05 or 0.1 N hcl acid in 70% ethanol

122
Q

color of non acid fast decolorizer

A

colorless

123
Q

color of non acid fast secondary staining

A

blue/green

124
Q

causative agent of tuberculosis and leprosy

A

Mycobacterium

125
Q

Used to identify myelin which stains blue

A

luxol fast blue

126
Q

Used to identify Other elements of the nervous system which is stains pink or violet

A

cresyl violet

127
Q

This is stain mainly used to evaluate the type and amount of extracellular material like collagen, fibrin, muscle and elastic fibers

A

trichrome stain

128
Q

It’s principle is smaller dye molecules will penetrate and stain a tissue element

A

Masson’s trichrome stain

129
Q

Collagen fibers stains

A

masson’s trichrome stain and van gieson

130
Q

elastic fibers stain

A

verhoeff’s stain, orcein stain, weigert’s fuschin stain, aldehyde fuschin

131
Q

reticular fibers stain

A

gordon & sweet’s stain and gomori’s stain

132
Q

muscle stain

A

mallory ptah,heidenhain’s iron hematoxylin and masson’s trichrome

133
Q

carbohydrates stain

A

periodic acid schiff, alcian blue, mucicarmine, colloidal iron stain

134
Q

amyloid stain

A

CRYSTAL VIOLET
CONGO RED
SIRIUS RED
THIOFLAVIN -t

135
Q

lipid stain

A

OIL RED O
SUDAN BLACK B
OSMIUM TETROXIDE

136
Q

nerve cell stain

A

NISSL STAIN
BIELSCHEWSKY STAIN
EAGER’S STAIN

137
Q

BONE stain

A

SCHMOR’S PICROTHIONINE
GOLDNER’S TRICHROME
SOLOCHROME

138
Q

melanin stain

A

MASSON FONTANA SILVER STAIN
SCHMORL’S FERRICYANIDE

139
Q

It is a genetic disorder which increase iron absorption and deposition in various organs

A

Hemochromatosis

140
Q

It is a versatile dye that colors nucle blue

A

toluidine blue

141
Q

in van gieson stain, the color of nuclei is

A

blue or black

142
Q

in van gieson stain, the color of cytoplasm, muscle, fibrin and RBC is

A

yellow

143
Q

in VG stain, the color of collagen is

A

red

144
Q

stain used in the diagnosis of acute lymphocytic leukemia

A

orcein stain

145
Q

Stain used to identify deposits of protein in tissue

A

congo red

146
Q

It is a versatile tool that used to distinguish elements of the extracellular matrix

A

alcian blue

147
Q

It is a histologic dye that is used primarily stain acidic union

A

Copper phthalocyanine dye

148
Q

It is a dye that is more soluble in fat than in water or alcohols

A

Oil red O

149
Q

What is the stain which principle is dilute mineral acid Hydrolysis releases ferric iron from protein bound tissue deposits

A

perl’s prussian blue

150
Q

What is the stain of perls prussian blue

A

potassium ferric ferrocyanide

histo lab (2 decks)

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