Porphryins and Iron

Question 1

Lead positioning blocks what?

Answer 1

heme synthesis between delta ALA and porphobilinogen

Question 2

Watson Schwartz test

Answer 2

Porphobilinogen
Urine + Ehrlich’s Reagent => + magenta color
Urobilinogen can cause false +
Eliminate urobilinogen through extraction
Urobilinogen is extracted into organic layer (chloroform or butanol)
Porphobilinogen goes into aqueous layer

Question 3

increased serum iron is from what?

Answer 3

Increased destruction of RBC
Ineffective RBC formation
Blocks in Heme synthesis such as lead poisoning
Increase in release of stored iron(hepatic destruction)
Increased intake of iron

Question 4

Increased Total Iron Binding Capacity is from what?

Answer 4

Late pregnancy
Iron deficiency anemia (compensation)
Acute hemorrhage
Acute destruction of liver cells

Question 5

normal iron ranges

Answer 5

55-160 ug/dl (M)
40-155 ug/dl (F)

Question 6

Total Iron Binding Capacity reference range

Answer 6

20-50%

Question 7

iron % saturation reference range

Answer 7

255-450 ug/dl

Question 8

Decreased Serum Iron is due to what?

Answer 8

Dietary deficiency or malabsorption
Loss of iron (blood loss or late pregnancy)
Impaired release of stored iron

Question 9

Decreased Total Iron Binding Capacity is due to what?

Answer 9

Decreased synthesis of Transferrin
–
Increased loss of protein as in renal disease

Question 10

Principle of Assays:
Total Serum Iron

Answer 10

Dissociate ferric iron from transferrin and reduce to ferrous form
Iron + Color Reagent => Color Change

Question 11

Principle of Assay:
TIBC

Answer 11

Saturate transferrin with ferric iron
Remove excess free iron from reaction tube
Measure iron as in total serum iron

Question 12

PERCENT SATURATION OF TIBC CALCULATION

Answer 12

% Saturation = Serum Iron/TIBC x 100

Question 13

Wilson’s Disease

Answer 13

Missing enzyme
Causes copper build-up in tissues
Pathology to soft tissue especially the liver