Polymerase Chain Reaction Flashcards
1
Q
Chromosome
A
Thread-like structure containing DNA
2
Q
What does PCR do?
A
PCR uses the enzyme DNA polymerase to replicate or amplify the DNA strand
3
Q
PCR step 1
A
Temperature increased to 95C to separate DNA strands
4
Q
PCR step 2
A
Primers introduced and temperature reduced to allow bonding (50C-60C)
5
Q
PCR step 3
A
DNA replication occurs using DNA polymerase (72C)
6
Q
PCR step 4
A
Double-stranded DNA produced consisting of a template and a new strand of DNA
7
Q
PCR step 5
A
Temperature increased to separate the strands and the cycle begins again
8
Q
Uses of PCR
A
- Forensics
- Diagnosis of diseases
- Paternity cases
- Various medical uses
